Major Topics

Clinical Proteomics and Metabolomics

Pietrowska M, Diehl HC, Mrukwa G, Kalinowska-Herok M, Gawin M, Chekan M, Elm J, Drazek G, Krawczyk A, Lange D, Meyer HE, Polanska J, Henkel C, Widlak P (2016): Molecular profiles of thyroid cancer subtypes: Classification based on features of tissue revealed by mass spectrometry imaging. Biochim Biophys Acta. doi: 10.1016/j.bbapap.2016.10.006 [Epub ahead of print]

https://www.ncbi.nlm.nih.gov/pubmed/27760391

Roś-Mazurczyk M, Wojakowska A, Marczak Ł, Polański K, Pietrowska M, Jelonek K, Domińczyk I, Hajduk A, Rutkowski T, Składowski K, Widłak P (2016): Ionizing radiation affects profile of serum metabolites: increased level of 3-hydroxybutyric acid in serum of cancer patients treated with radiotherapy. Acta Biochim Pol. doi:10.18388/abp.2016_1301 [Epub ahead of print]

https://www.ncbi.nlm.nih.gov/pubmed/27815965

Walaszczyk A, Pietrowska M, Wojakowska A, Abramowicz A, Chmura A, Masłyk B, Rodziewicz P, Polańska J, Behrendt K, Nowicka E, Tarnawski R, Widłak P (2016) Therapy-related changes in serum proteome patterns of early stage breast cancer patients with different outcome. Protein Pept Lett. [Epub ahead of print]

https://www.ncbi.nlm.nih.gov/pubmed/27894246

Roś-Mazurczyk M, Jelonek K, Pietrowska M, Zagdański A, Suchwałko A, Jastrząb T, Polańska J, Chawińska E, Majewski W, Domińczyk I, Rutkowski T, Miszczyk L, Składowski K, Widłak P (2016): Radiotherapy-induced changes in serum lipid profile of patients with prostate or head and neck cancer. J J Rad Oncol. 2016, 3(2): 030.

http://radiationoncology.jacobspublishers.com/images/Rad_Onco/J_J_Rad_Oncol_3_2_030.pdf

Widlak P, Pietrowska M, Polanska J, Marczyk M, Ros-Mazurczyk M, Dziadziuszko R, Jassem J, Rzyman W (2016): Serum mass profile signature as a biomarker of early lung cancer. Lung Cancer 99:46-52.

https://www.ncbi.nlm.nih.gov/pubmed/27565913

Pietrowska M, Gawin M, Polańska J, Widłak P (2016): Tissue fixed with formalin and processed without paraffin embedding is suitable for imaging of both peptides and lipids by MALDI-IMS. Proteomics 16:1670-1677.

https://www.ncbi.nlm.nih.gov/pubmed/27001204

Widlak P, Mrukwa G, Kalinowska M, Pietrowska M, Chekan M, Wierzgon J, Gawin M, Drazek G, Polanska J (2016): Detection of molecular signatures of oral squamous cell carcinoma and normal epithelium - application of a novel methodology for unsupervised segmentation of imaging mass spectrometry data. Proteomics 16:1613-1621.

https://www.ncbi.nlm.nih.gov/pubmed/27168173

Szymańska K, Pietrowska M, Kocurek J, Maresz K, Koreniuk A, Mrowiec-Białoń J, Widłak P, Magner E, Jarzębski A (2016): Low back-pressure hierarchically structured multichannel microfluidic bioreactors for rapid protein digestion - proof of concept. Chem Eng J  287: 148-54.

http://dx.doi.org/10.1016/j.cej.2015.10.120

Gadher SJ, Marczak Ł, Łuczak M, Stobiecki M, Widlak P, Kovarova H (2016): Proteomic landscape in Central and Eastern Europe: the 9th Central and Eastern European Proteomic Conference, Poznań, Poland. Expert Rev Proteomics 13: 5-7. 

http://www.ncbi.nlm.nih.gov/pubmed/26558656

Liśkiewicz AD, Kasprowska D, Wojakowska A, Polański K, Lewin-Kowalik J, Kotulska K, Jędrzejowska-Szypułka H (2016) Long-term high fat ketogenic diet promotes renal tumor growth in a rat model of tuberous sclerosis. Sci Rep. 6: 21807.

https://www.ncbi.nlm.nih.gov/pubmed/26892894

Tóth E, Vékey K, Ozohanics O, Jekő A, Dominczyk I, Widlak P, Drahos L (2016): Changes of protein glycosylation in the course of radiotherapy. J Pharm Biomed Anal 118: 380-6.

http://www.ncbi.nlm.nih.gov/pubmed/26609677

Abramowicz A, Wojakowska A, Gdowicz-Klosok A, Polanska J, Rodziewicz P, Polanowski P, Namysl-Kaletka A, Pietrowska M, Wydmanski J, Widlak P (2015): Identification of serum proteome signatures of locally advanced and metastatic gastric cancer: a pilot study. J Transl Med 13: e304.

http://www.ncbi.nlm.nih.gov/pubmed/26376850

Pietrowska M, Jelonek K, Polanska J, Wojakowska A, Marczak L, Chawinska E, Chmura A, Majewski W, Miszczyk L, Widlak P (2015): Partial-body irradiation in patients with prostate cancer treated with IMRT has little effect on the composition of serum proteome. Proteomes 3: 117-31.

http://www.mdpi.com/2227-7382/3/3/117 

Polanski A, Marczyk M, Pietrowska M, Widlak P, Polanska J (2015): Signal partitioning algorithm for highly efficient Gaussian Mixture Modeling in mass spectrometry. PLoS One 10: e0134256.

http://www.ncbi.nlm.nih.gov/pubmed/26230717

Widłak P, Jelonek K, Wojakowska M, Pietrowska M, Polanska J, Marczak L, Miszczyk L, Składowski K (2015) Serum proteome signature of radiation response: upregulation of inflammation-related factors, and downregulation of apolipoproteins and coagulation factors in cancer patients subjected to radiotherapy – a pilot study. Int J Radiat Oncol Biol Phys 92: 1108-15.

http://www.ncbi.nlm.nih.gov/pubmed/26031365

Wojakowska A, Chekan M, Marczak Ł, Polanski K, Lange D, Pietrowska M, Widlak P (2015) Detection of metabolites discriminating subtypes of thyroid cancer: molecular profiling of FFPE samples using the GC/MS approach. Mol Cell Endocrinol 417: 149-57.

http://www.ncbi.nlm.nih.gov/pubmed/26415588

Wojakowska A, Chekan M, Widłak P, Pietrowska M (2015) Application of metabolomics in thyroid cancer research. Int J Endocrinol 2015:258763.

http://www.ncbi.nlm.nih.gov/pubmed/25972898

Wojakowska A, Marczak Ł, Jelonek K, Polanski K, Widlak P, Pietrowska M (2015): An optimized method of metabolite extraction from formalin-fixed paraffin-embedded tissue for GC/MS analysis. PLoS One 10:e0136902.

http://www.ncbi.nlm.nih.gov/pubmed/26348873

Pietrowska M, Jelonek K, Michalak M, Roś M, Rodziewicz P, Chmielewska K, Polański K, Polańska J, Gdowicz-Kłosok A, Giglok M, Suwiński R, Tarnawski R, Dziadziuszko R, Rzyman W, Widłak P (2014): Identification of serum proteome components associated with progression of non-small cell lung cancer. Acta Biochim. Pol. 61: 325-331.

http://www.ncbi.nlm.nih.gov/pubmed/24872961

Jelonek K, Pietrowska M, Roś M, Zagdański A, Suchwałko A, Polańska J, Marczyk M, Rutkowski T, Składowski K, Clench MR, Widlak P (2014): Radiation-induced changes in serum lipidome of head and neck cancer patients. Int. J. Mol. Sci. 15: 6609-6624.

http://www.ncbi.nlm.nih.gov/pubmed/24747595

Widłak P, Pietrowska M, Polańska J, Rutkowski T, Jelonek K, Kalinowska-Herok M, Gdowicz-Kłosok A, Wygoda A, Tarnawski R, Składowski K (2013): Radiotherapy-related changes in serum proteome patterns of head and neck cancer patients; the effect of low and medium doses of radiation delivered to large volumes of normal tissue. J. Transl. Med. 11: 299.

http://www.ncbi.nlm.nih.gov/pubmed/24304975

Kalinowska-Herok M, Pietrowska M, Walaszczyk A, Widlak P (2013): MALDI Imaging Mass Spectrometry – A Novel Approach in Biomedical Research of Tissues. Curr. Proteomics 10(2): 76-82.

http://www.ingentaconnect.com/content/ben/cp/2013/00000010/00000002/art00002

Jelonek K, Roś M, Pietrowska M, Widłak P (2013): Cancer biomarkers and mass spectrometry-based analyses of phospholipids in body fluids. Clin. Lipidol. 8: 137-150.

http://www.futuremedicine.com/doi/abs/10.2217/clp.12.79

Pietrowska M, Widłak P (2012) MALDI-MS-based profiling of serum proteome: detection of changes related to progression of cancer and response to anticancer treatment. Int. J. Proteomics 2012:926427.

http://www.ncbi.nlm.nih.gov/pubmed/22900176

Pietrowska M, Polańska J, Suwiński R, Wideł M, Rutkowski T, Marczyk M, Marczak L, Polański A, Widłak P (2012): Comparison of peptide cancer signatures identified by mass spectrometry in serum of patients with head and neck, lung, and colorectal cancers. Int. J. Oncol.40: 148-156.

http://www.ncbi.nlm.nih.gov/pubmed/21894432

Pietrowska M, Polańska J, Walaszczyk A, Wygoda A, Rutkowski T, Składowski K, Marczak L, Stobiecki M, Marczyk M, Polański A, Widłak P (2011): Association between plasma proteome profiles analysed by mass spectrometry, a lymphocyte-based DNA-break repair assay and radiotherapy-induced acute mucosal reaction in head and neck cancer patients. Int. J. Radiat. Biol. 87: 711-719.

http://www.ncbi.nlm.nih.gov/pubmed/21351848

Widłak P, Pietrowska M, Wojtkiewicz K, Rutkowski T, Wygoda A, Marczak L, Marczyk M, Polańska J, Walaszczyk A, Domińczyk I, Składowski K, Stobiecki M, Polański A (2011): Radiation-related changes in serum proteome profiles detected by mass spectrometry in blood of patients treated with radiotherapy due to larynx cancer. J. Radiat. Res. 52: 575-581.

http://www.ncbi.nlm.nih.gov/pubmed/21768750

Pietrowska M, Polańska J, Marczak Ł, Behrendt K, Nowicka E, Stobiecki M, Polański A, Tarnawski R, Widłak P (2010): Mass spectrometry-based analysis of therapy-related changes in serum proteome patterns of patients with early stage breast cancer. J. Translat. Med. 8: e66.

http://www.ncbi.nlm.nih.gov/pubmed/20618994

Pietrowska M, Marczak Ł, Polańska J, Nowicka E, Behrendt K, Tarnawski R, Stobiecki M, Polański A, Widłak P (2010): Optimizing of MALDI-ToF-based low-molecular-weight serum proteome pattern analysis in detection of breast cancer patients; the effect of albumin removal on classification performance. Neoplasma 57: 537-544.

http://www.ncbi.nlm.nih.gov/pubmed/20845992

Pietrowska M, Marczak Ł, Polańska J, Behrendt K, Nowicka E, Walaszczyk A, Chmura A, Deja R, Stobiecki M, Polański A, Tarnawski R, Widłak P (2009): Mass spectrometry-based serum proteome pattern analysis in molecular diagnostics of early stage breast cancer. J. Translat. Med. 7: e60.

http://www.ncbi.nlm.nih.gov/pubmed/19594898

also:

Jelonek K, Pietrowska M, Widłak P (2016) Główne zmiany proteomu i metabolomu surowicy obserwowane w krwi osób poddanych radioterapii dotyczą wskaźników stanu zapalnego i odpowiedzi immunologicznej odzwierciedlających systemową toksyczność promieniowania jonizującego. Onkologia I Radioterapia 3, 24-29

Kalinowska-Herok M, Roś M, Widłak P. (2013): Molekularne marginesy guza nowotworowego. Nowotwory – J. Oncol. 63: 28-34.

Hanus J, Jelonek K, Pietrowska P (2011): Wykorzystanie spektrometrii mas do analizy modyfikacji nukleotydów i adduktów DNA.Wiadomości Chemiczne 65: 191-205.

Chmura A, Deja R, Mrochem-Kwarciak J, Masłyk B, Polańska J, Pietrowska M, Ponge L, Behrendt K, Nowicka E, Tarnawski R, Widłak P (2011): Analiza zmian stężenia osteopontyny w osoczu krwi chorych leczonych z powodu nisko zaawansowanego raka piersi. Onkologia Info 8: 4-10.

Wojtkiewicz K, Marczyk M, Polańska J, Polański A, Marczak Ł, Stobiecki M, Pietrowska M, Domińczyk I, Behrendt K, Nowicka E, Tarnawski R, Widłak P (2010): Wykorzystanie spektrometrii mas do analizy proteomu surowicy chorych na raka piersi. Onkologia Info 7: 40-47.

Pietrowska M (2009): Tumor markers studied with proteomic methods in blood and serum plasma. Biotechnologia 2009(2): 39-53.

Plechawska M, Polańska J, Polański A, Pietrowska M, Tarnawski R , Widłak P, Stobiecki M, Marczak Ł (2009): Analyze of MALDI-TOF Proteomic Spectra with Usage of Mixture of Gaussian Distributions. (in:) Advances in Soft Computing , 2009, Volume 59 , Man-Machine Interactions (eds. KA Cyran, S Kozielski , JF Peters, U Stanczyk, A Wakulicz-Deja) , pp. 113-120

Polańska J, Widłak P, Rzeszowska-Wolny J, Kimmel M, Polański A (2007): Gaussian mixture decomposition of time-course DNA microarray data. w: Mathematical Modeling of Biological Systems: Cellular Biophysics, Regulatory Network, Development, Biomedicine, and Data Analysis, volume I, ed. A. Deutsch; Birkhäuser & Springer, Cambridge, str.: 351-359.

Pietrowska M, Marczak Ł, Widłak P (2007): Proteomika kliniczna – wykorzystanie metod spektrometrii mas do analizy proteomu surowicy krwi w diagnostyce chorób nowotworowych. w: Na Pograniczu Chemii i Biologii, tom. XVII, str. 93-109. Wyd. UAM, Poznań.

 

Exosomes

Jelonek K, Widlak P, Pietrowska M (2016): The Influence of Ionizing Radiation on Exosome Composition, Secretion and Intercellular Communication. Protein Pept Lett. 23: 656-663.

https://www.ncbi.nlm.nih.gov/pubmed/27117741

Abramowicz A, Widlak P, Pietrowska M (2016): Proteomic analysis of exosomal cargo: the challenge of high purity vesicle isolation. Mol Biosyst. 12:1407-1419.

https://www.ncbi.nlm.nih.gov/pubmed/27030573

Jelonek K, Wojakowska A, Marczak L, Muer A, Tinhofer-Keilholz I, Lysek-Gladysinska M, Widlak P, Pietrowska M (2015): Ionizing radiation affects protein composition of exosomes secreted in vitro from head and neck squamous cell carcinoma. Acta Biochim Pol 62: 265-272.

http://www.ncbi.nlm.nih.gov/pubmed/26098714

 

HSF1- and NF-κB-dependent signaling pathways

Szoltysek K, Walaszczyk A, Janus P, Kimmel M, Widlak P (2017): Irradiation with UV-C inhibits TNF-α-dependent activation of the NF-κB pathway in a mechanism potentially mediated by reactive oxygen species. Genes Cells. 22: 45-58.

https://www.ncbi.nlm.nih.gov/pubmed/27976481

Jonak K, Kurpas M, Szoltysek K, Janus P, Abramowicz A, Puszynski K (2016): A novel mathematical model of ATM/p53/NF- κB pathways points to the importance of the DDR switch-off mechanisms. BMC Syst Biol. 10: 75.

https://www.ncbi.nlm.nih.gov/pubmed/27526774

Janus P, Stokowy T, Jaksik R, Szoltysek K, Handschuh L, Podkowinski J, Widlak W, Kimmel M, Widlak P (2015): Cross talk between cytokine and hyperthermia-induced pathways: identification of different subsets of NF-κB-dependent genes regulated by TNFα and heat shock. Mol Genet Genomics 290:1979-90.

http://www.ncbi.nlm.nih.gov/pubmed/25944781

Toma-Jonik A, Widlak W, Korfanty J, Cichon T, Smolarczyk R, Gogler-Piglowska A, Widlak P, Vydra N (2015): Active heat shock transcription factor 1 supports migration of the melanoma cells via vinculin down-regulation. Cell Signal 27:394-401.

http://www.ncbi.nlm.nih.gov/pubmed/25435429

Korfanty J, Stokowy T, Widlak P, Gogler-Piglowska A, Handschuh L, Podkowiński J, Vydra N, Naumowicz A, Toma-Jonik A, Widlak W (2014): Crosstalk between HSF1 and HSF2 during the heat shock response in mouse testes. Int J Biochem Cell Biol. 57: 76-83.

http://www.ncbi.nlm.nih.gov/pubmed/25450459

Janus P, Pakuła-Cis M, Kalinowska-Herok M, Kashchak N, Szołtysek K, Pigłowski W, Widłak W, Kimmel M, Widłak P (2011): NF- kappa B signaling pathway is inhibited by heat shock independently of active transcription factor HSF1 and increased levels of inducible heat shock proteins. Genes Cells 16: 1168-1175.

http://www.ncbi.nlm.nih.gov/pubmed/22077664

Szołtysek K, Pietranek K, Kalinowska-Herok M, Pietrowska M, Kimmel M, Widłak P (2008): TNF a -induced activation of NF kappa B protects against UV-induced apoptosis specifically in p53-proficient cells. Acta Biochim. Polon 55: 741-748.

http://www.ncbi.nlm.nih.gov/pubmed/19023456

Widłak W, Vydra N, Malusecka E, Dudaladava V, Winiarski B, Ścieglinska D, Widłak P (2007): Heat shock transcription factor 1 down-regulates spermatocyte-specific 70 kDa heat shock protein expression prior to the induction of apoptosis in mouse testes. Genes Cells12: 487-499.

http://www.ncbi.nlm.nih.gov/pubmed/17397396

Vydra N, Małusecka E, Jarząb M, Lisowska K, Głowala-Kosińska M, Benedykt K, Widłak P, Krawczyk Z, Widłak W (2006): Spermatocyte-specific expression of constitutively active heat shock factor 1 induces HSP70i-resistant apoptosis in male germ cells. Cell Death Differ .13: 212-222.

http://www.ncbi.nlm.nih.gov/pubmed/16151457

also:

Szołtysek K, Janus P, Widłak (2011): Komórkowa ścieżka sygnałowa zależna od czynnika transkrypcyjnego NF-kB i jej współzależności ze szlakami p53 i HSF1. Postępy Biologii Komórki 38: 159-175.

Widłak P, Gramatyka M, Kimmel M (2014): Crosstalk between stress-induced NF-κB, p53 and HSF1 signaling pathways – review. Preprints of the 19th World Congress of the International Federation of Automatic Control: 11518-11523.

 

Apoptotic nucleases and chromatin fragmentation

Hanus J, Kalinowska-Herok M, Widłak P (2010): Identification of novel putative regulators of the major apoptotic nuclease DNA Fragmentation Factor. Acta Biochim. Polon57: 521-527.

http://www.ncbi.nlm.nih.gov/pubmed/21152448

Widłak P, Garrard WT (2009): Roles of the major apoptotic nuclease - DNA Fragmentation Factor - in biology and disease. Cell. Mol. Life Sci 66: 263-274.

http://www.ncbi.nlm.nih.gov/pubmed/18810317

Kalinowska-Herok M, Widłak P (2008): High Mobility Group proteins stimulate DNA cleavage by apoptotic endonuclease DFF40/CAD due to HMG-box interactions with DNA. Acta Biochim. Polon 55: 21-26.

http://www.ncbi.nlm.nih.gov/pubmed/18239742

Hanus J, Kalinowska-Herok M, Widłak P (2008): The major apoptotic endonuclease DFF40/CAD is a deoxyribose-specific and double-strand-specific enzyme. Apoptosis 13: 377-382.

http://www.ncbi.nlm.nih.gov/pubmed/18283539

Xiao F, Widłak P, Garrard WT (2007): Engineered apoptotic nucleases for chromatin research. Nucl. Acids Res 35, e93.

http://www.ncbi.nlm.nih.gov/pubmed/17626049

Sikora E, Bielak-Żmijewska A, Magalska A, Piwocka K, Mosieniak G, Kalinowska M, Widłak P, Cymerman IA, Bujnicki JM (2006): Curcumin induces caspase-3-dependent apoptotic pathway but inhibits DNA fragmentation factor 40/caspase-activated DNase endonuclease in human Jurkat cells . Mol. Cancer Ther. 5: 927-934.

http://www.ncbi.nlm.nih.gov/pubmed/16648563

Widłak P, Garrard WT (2006): The apoptotic endonuclease DFF40/CAD is inhibited by RNA, heparin and other polyanions. Apoptosis 11: 1331-1337.

http://www.ncbi.nlm.nih.gov/pubmed/16699957

Widłak P, Garrard WT (2006): Unique features of the apoptotic endonuclease DFF40/CAD relative to micrococcal nuclease as a structural probe for chromatin. Biochem. Cell Biol 84: 405-410.

http://www.ncbi.nlm.nih.gov/pubmed/16936813

Widłak P, Garrard WT (2005): Discovery, regulation, and action of the major apoptotic nucleases DFF40/CAD and endonuclease G. J. Cell. Biochem 94: 1078-1087.

http://www.ncbi.nlm.nih.gov/pubmed/15723341

Widłak P, Kalinowska M , Parseghian MH, Lu X, Hansen JC, Garrard WT (2005): The histone H1 C-terminal domain binds to the apoptotic nuclease, DNA Fragmentation Factor (DFF40/CAD) and stimulates DNA cleavage. Biochemistry,44: 7871-7878.

http://www.ncbi.nlm.nih.gov/pubmed/15910001

Kalinowska M, Garncarz W, Pietrowska M, Garrard WT, Widłak P (2005): Regulation of the human apoptotic DNase/RNase EndoG; involvement of Hsp70 and ATP. Apoptosis 10: 821-830.

http://www.ncbi.nlm.nih.gov/pubmed/16133872

Widłak P, Łanuszewska. J, Cary RB, Garrard WT (2003): Subunit structures and stoichiometries of human DFF proteins before and after induction of apoptosis. J. Biol. Chem. 278: 26915-26922.

http://www.ncbi.nlm.nih.gov/pubmed/12748178

Widłak P, Palyvoda O, Kumala S, Garrard WT (2002): Modeling apoptotic chromatin condensation in normal cell nuclei; requirement for intranuclear mobility and actin involvement. J. Biol. Chem 277: 21683-21690.

http://www.ncbi.nlm.nih.gov/pubmed/11927586

Widłak P, Li LY, Wang X, Garrard WT (2001): Action of recombinant human apoptotic endonuclease G on naked DNA and chromatin substrates; cooperation with exonuclease and DNaseI. J. Biol. Chem 276: 48404–48409.

http://www.ncbi.nlm.nih.gov/pubmed/11606588

Widłak P, Garrard WT (2001): Ionic and cofactor requirements for the activity of the apoptotic endonuclease DFF40/CAD. Mol. Cell. Biochem 218: 125-130.

http://www.ncbi.nlm.nih.gov/pubmed/11330826

Widłak P, Li P, Wang X, Garrard W (2000): Cleavage preferences of the apoptotic endonuclease DFF40 (Caspase-activated DNase or Nuclease) on naked DNA and chromatin substrates. J. Biol. Chem. 275: 8226-8232.

http://www.ncbi.nlm.nih.gov/pubmed/10713148

Widłak P (2000): DFF40/CAD hypersensitive sites are potentially involved in high molecular weight DNA fragmentation during apoptosis.Cell. Mol. Biol. Lett 5: 373-379.

http://www.cmbl.org.pl/pdf/Vol5_p373-379.pdf

Widłak P. (2000): The DFF40/CAD endonuclease and its role in apoptosis. Acta Biochim. Polon 47: 1037-1044.

http://www.ncbi.nlm.nih.gov/pubmed/11996094

also:

Widłak P (2000): Mechanizmy fragmentacji DNA i kondensacji chromatyny w komórkach ulegających apoptozie. Postępy Biologii Komórki27: 583-597.

 

Damaged DNA-binding proteins

Widłak P, Pietrowska M, Łanuszewska J (2006): The role of chromatin proteins in DNA damage recognition and repair. Histochem Cell Biol. 125: 119-126.

http://www.ncbi.nlm.nih.gov/pubmed/16163486

Pietrowska M, Kołodziejczyk I, Widłak P (2006): Mitochondrial transcription factor A is the major protein in rodent hepatocytes that recognizes DNA lesions induced by N-acetoxy-acetylaminofluorene. Acta Biochim. Polon 53: 777-782.

http://www.ncbi.nlm.nih.gov/pubmed/17143337

Pietrowska M, Widłak P (2005): Characterization of a novel protein that specifically binds to DNA modified by N-acetoxy-acetylaminofluorene and cis -diammine-dichloro-platinum. Acta Biochim. Polon ., 52: 867-874.

http://www.ncbi.nlm.nih.gov/pubmed/15940347

Łanuszewska J, Widłak P (2004): The truncation of Ku86 in human lymphocytes. Cancer Lett 205: 197-205.

http://www.ncbi.nlm.nih.gov/pubmed/15036652

Łanuszewska J, Cudak A, Rzeszowska-Wolny J, Widłak P (2000) Detection of damage-recognition proteins from human lymphocytes.Acta Biochim. Polon 47: 443-450.

http://www.ncbi.nlm.nih.gov/pubmed/11051209

Łanuszewska J, Widłak P (2000): High mobility group 1 and 2 proteins bind preferentially to DNA that contains bulky adducts induced by benzo(a)pyrene diol epoxide and N-acetoxy-acetylaminofluorene. Cancer Lett 158: 17-25.

http://www.ncbi.nlm.nih.gov/pubmed/10940504

Pietrowska M, Łanuszewska J, Walter Z, Rzeszowska-Wolny J, Widłak P (2000): Detection and characterization of rat protein recognizing DNA damaged by N-acetoxy-acetylaminofluorene. Cell. Mol. Biol. Lett 5: 423-431.

http://www.cmbl.org.pl/pdf/Vol5_p423-431.pdf

also:

Łanuszewska J, Widłak P (2005): Udział białek chromatyny w rozpoznawaniu i naprawie uszkodzeń DNA. w: Na Pograniczu Chemii i Biologii, tom. XII, str. 269-287. Wyd. UAM, Poznań.

Łanuszewska J, Widłak P (2003): Białka detektorowe rozpoznające pęknięcia nici DNA i ich udział w mechanizmach komórkowej odpowiedzi na stres. Postępy Biochemii 49: 229-238.

Widłak P (2000): Białka rozpoznające i wiążące się z uszkodzonym DNA; udział w mechanizmach naprawy DNA i kancerogenezie. Postępy Biochemii 46: 198-206.

 

Other Topics

DNA damage and response to damaging factors

Gramatyka M, Widłak P (2015): Neither resveratrol nor metformin protects human cardiomyocytes against toxicity of epirubicin and radiation in-vitro. Medical Science, 2015,17(67):1-10.

http://discoveryjournals.com/medicalscience/current_issue/v17/n67/A1.pdf

Jelonek K, Walaszczyk A, Gabryś D, Pietrowska M, Kanthou C, Widłak P (2011): Cardiac endothelial cells isolated from mouse heart - a novel model for radiobiology. Acta Biochim. Polon 58 : 397-404.

http://www.ncbi.nlm.nih.gov/pubmed/21887413

Jelonek K, Gdowicz-Kłosok A, Pietrowska P, Borkowska M, Korfanty J, Rzeszowska-Wolny J, Widłak P (2010): Association between single-nucleotide polymorphisms of selected genes involved in the response to DNA damage and risk of colon, head and neck, and breast cancers in a Polish population. J. Appl. Genet 51 : 343-352.

http://www.ncbi.nlm.nih.gov/pubmed/20720310

Horak S, Olejek A, Widłak P (2007): Sperm DNA adducts impair fertilization during ICSI but not during IVF. Folia Histochem. Cytobiol. 45 , suppl. 1: S99-S104.

http://www.ncbi.nlm.nih.gov/pubmed/18292844

Horak S, Polańska J, Widłak P (2003): High levels of bulky DNA adducts in human sperm correlate with impaired fertility. Acta Biochim. Polon 50 : 197-203.

http://www.ncbi.nlm.nih.gov/pubmed/12673360

Horak S, Polańska J, Widłak P (2003): Bulky DNA adducts in human sperm: relationship with fertility, semen quality, smoking, and environmental factors. Mutat. Res. 537 : 53-65.

http://www.ncbi.nlm.nih.gov/pubmed/12742507

Rzeszowska-Wolny J, Polańska J, Pietrowska M, Palyvoda O, Jaworska J, Butkiewicz D, Hancock R (2005):. Influence of polymorphisms in DNA repair genes XPD, XRCC1 and MGMT on DNA damage induced by gamma radiation and its repair in lymphocytes in vitro. Radiat. Res164:132-140.

http://www.ncbi.nlm.nih.gov/pubmed/16038584

Walaszczyk A, Pietrowska M (2009): Kardiotoksyczność promieniowania jonizującego. w: Na Pograniczu Chemii i Biologii, tom. XXII, str. 347-369. Wyd. UAM, Poznań.

Widłak P (2002): Struktura chromatyny a powstawanie i naprawa uszkodzeń DNA. Kosmos 51: 5-12.

Widłak P (2000): The DNA damage-induced cell cycle checkpoints. J. Theor. Med2: 273-243.

Widłak P (2000): Naprawa dwuniciowych pęknięć DNA indukowanych przez promieniowanie jonizujące. Postępy Higieny i Medycyny Doświadczalnej 54: 133-148.

 

Chromatin and gene regulation

Piccinni E, Chełstowska A, Hanus J, Widłak P , Loreti S, Tata AM, Augusti-Tocco G, Bianchi MM, Negri R (2011): Direct interaction of Gas41 and Myc encoded by amplified genes in nervous system tumours. Acta Biochim. Polon58: 529-534.

http://www.ncbi.nlm.nih.gov/pubmed/22068108

Liu Z, Widłak P, Zou Y, Xiao F, Oh M, Li S, Chang MY, Shay JW, Garrard WT (2006): A recombination silencer that specifies heterochromatin positioning and Ikaros association in the immunoglobulin k locus. Immunity 24: 405-415.

http://www.ncbi.nlm.nih.gov/pubmed/16618599

Widłak W, Widłak P (2004): MAR/SAR elements flank the rat hst70 gene transcription unit. Cell. Mol. Biol. Lett 9: 123-133.

http://www.ncbi.nlm.nih.gov/pubmed/15048156

Fedorov A, Lukyanov D, Rogoliński J, Widłak P, Podgornaya O, Rzeszowska-Wolny J (2004): The nuclear protein p30 interacts with a nuclear matrix attachment region from the rat genome. Cell. Mol. Biol. Lett 9: 153-165.

http://www.ncbi.nlm.nih.gov/pubmed/15048159

Widłak P (2004): DNA microarrays, a novel approach in studies of chromatin structure. Acta Biochim. Polon 51: 1-8.

http://www.ncbi.nlm.nih.gov/pubmed/15094821

Weil MR, Widłak P, Minna JD, Garner HR (2004) Global survey of chromatin accessibility using DNA microarrays. Genome Res 14: 1374-1381.

http://www.ncbi.nlm.nih.gov/pubmed/15231753

Widłak P, Fujarewicz K (2003): The analysis of chromatin condensation state and transcriptional activity using DNA microarrays. Journal of Medical Informatics and Technologies. 6: IP13-IP19.