Major Topics

Clinical Proteomics and Metabolomics

Gawin M, Kurczyk A, Stobiecka E, Frątczak K, Polańska J, Pietrowska M, Widłak P (2019): Molecular Heterogeneity of Papillary Thyroid Cancer: Comparison of Primary Tumors and Synchronous Metastases in Regional Lymph Nodes by Mass Spectrometry Imaging. Endocr Pathol. 30: 250-62.

Bednarczyk K, Gawin M, Chekan M, Kurczyk A, Mrukwa G, Pietrowska M, Polanska J, Widlak P (2019): Discrimination of normal oral mucosa from oral cancer by mass spectrometry imaging of proteins and lipids. J Mol Histol. 50: 1-10.

Pietrowska M, Wlosowicz A, Gawin M, Widlak P (2019): MS-Based Proteomic Analysis of Serum and Plasma: Problem of High Abundant Components and Lights and Shadows of Albumin Removal. Adv Exp Med Biol. 1073: 57-76.

Jelonek K, Widłak P (2018): Metabolome-based biomarkers: their potential role in the early detection of lung cancer. Contemp Oncol (Pozn). 22: 135-40.

Wojakowska A, Cole LM, Chekan M, Bednarczyk K, Maksymiak M, Oczko-Wojciechowska M, Jarząb B, Clench MR, Polańska J, Pietrowska M, Widlak P (2018): Discrimination of papillary thyroid cancer from non-cancerous thyroid tissue based on lipid profiling by mass spectrometry imaging. Endokrynol Pol. 69: 2-8.

Gawin M, Wojakowska A, Pietrowska M, Marczak Ł, Chekan M, Jelonek K, Lange D, Jaksik R, Gruca A, Widłak P (2017): Proteome profiles of different types of thyroid cancers. Mol Cell Endocrinol472: 68-79.

Pietrowska M, Diehl HC, Mrukwa G, Kalinowska-Herok M, Gawin M, Chekan M, Elm J, Drazek G, Krawczyk A, Lange D, Meyer HE, Polanska J, Henkel C, Widlak P (2017): Molecular profiles of thyroid cancer subtypes: Classification based on features of tissue revealed by mass spectrometry imaging. Biochim Biophys Acta. 1865: 837-45.

Roś-Mazurczyk M, Wojakowska A, Marczak Ł, Polański K, Pietrowska M, Jelonek K, Domińczyk I, Hajduk A, Rutkowski T, Składowski K, Widłak P (2017): Ionizing radiation affects profile of serum metabolites: increased level of 3-hydroxybutyric acid in serum of cancer patients treated with radiotherapy. Acta Biochim Pol. 64: 189-93.

Jelonek K, Pietrowska M, Widlak P (2017): Systemic effects of ionizing radiation at the proteome and metabolome levels in the blood of cancer patients treated with radiotherapy: the influence of inflammation and radiation toxicity. Int J Radiat Biol. 93: 683-96.

Roś-Mazurczyk M, Jelonek K, Marczyk M, Binczyk F, Pietrowska M, Polanska J, Dziadziuszko R, Jassem J, Rzyman W, Widlak P (2017): Serum lipid profile discriminates patients with early lung cancer from healthy controls. Lung Cancer 112: 69-74.

Roś-Mazurczyk M, Wojakowska A, Marczak Ł, Polański K, Pietrowska M, Polanska J, Dziadziuszko R, Jassem J, Rzyman W, Widlak P (2017): Panel of serum metabolites discriminates cancer patients and healthy participants of lung cancer screening - a pilot study. Acta Biochim Pol. 64: 513-8.

Walaszczyk A, Pietrowska M, Wojakowska A, Abramowicz A, Chmura A, Masłyk B, Rodziewicz P, Polańska J, Behrendt K, Nowicka E, Tarnawski R, Widłak P (2017) Therapy-related changes in serum proteome patterns of early stage breast cancer patients with different outcome. Protein Pept Lett. 24: 37-45.

Roś-Mazurczyk M, Jelonek K, Pietrowska M, Zagdański A, Suchwałko A, Jastrząb T, Polańska J, Chawińska E, Majewski W, Domińczyk I, Rutkowski T, Miszczyk L, Składowski K, Widłak P (2016): Radiotherapy-induced changes in serum lipid profile of patients with prostate or head and neck cancer. J J Rad Oncol. 3(2): 030.

Widlak P, Pietrowska M, Polanska J, Marczyk M, Ros-Mazurczyk M, Dziadziuszko R, Jassem J, Rzyman W (2016): Serum mass profile signature as a biomarker of early lung cancer. Lung Cancer 99: 46-52.

Pietrowska M, Gawin M, Polańska J, Widłak P (2016): Tissue fixed with formalin and processed without paraffin embedding is suitable for imaging of both peptides and lipids by MALDI-IMS. Proteomics 16: 1670-7.

Widlak P, Mrukwa G, Kalinowska M, Pietrowska M, Chekan M, Wierzgon J, Gawin M, Drazek G, Polanska J (2016): Detection of molecular signatures of oral squamous cell carcinoma and normal epithelium - application of a novel methodology for unsupervised segmentation of imaging mass spectrometry data. Proteomics 16: 1613-21.

Szymańska K, Pietrowska M, Kocurek J, Maresz K, Koreniuk A, Mrowiec-Białoń J, Widłak P, Magner E, Jarzębski A (2016): Low back-pressure hierarchically structured multichannel microfluidic bioreactors for rapid protein digestion - proof of concept. Chem Eng J. 287: 148-54.

Gadher SJ, Marczak Ł, Łuczak M, Stobiecki M, Widlak P, Kovarova H (2016): Proteomic landscape in Central and Eastern Europe: the 9th Central and Eastern European Proteomic Conference, Poznań, Poland. Expert Rev Proteomics. 13: 5-7.

Liśkiewicz AD, Kasprowska D, Wojakowska A, Polański K, Lewin-Kowalik J, Kotulska K, Jędrzejowska-Szypułka H (2016): Long-term high fat ketogenic diet promotes renal tumor growth in a rat model of tuberoussclerosis. Sci Rep. 6: 21807.

Tóth E, Vékey K, Ozohanics O, Jekő A, Dominczyk I, Widlak P, Drahos L (2016): Changes of protein glycosylation in the course of radiotherapy. J Pharm Biomed Anal. 118: 380-6.

Abramowicz A, Wojakowska A, Gdowicz-Klosok A, Polanska J, Rodziewicz P, Polanowski P, Namysl-Kaletka A, Pietrowska M, Wydmanski J, Widlak P (2015): Identification of serum proteome signatures of locally advanced and metastatic gastric cancer: a pilot study. J Transl Med. 13: e304.

Pietrowska M, Jelonek K, Polanska J, Wojakowska A, Marczak L, Chawinska E, Chmura A, Majewski W, Miszczyk L, Widlak P (2015): Partial-body irradiation in patients with prostate cancer treated with IMRT has little effect on the composition of serum proteome. Proteomes 3: 117-31.

Polanski A, Marczyk M, Pietrowska M, Widlak P, Polanska J (2015): Signal partitioning algorithm for highly efficient Gaussian Mixture Modeling in mass spectrometry. PLoS One 10: e0134256.

Widłak P, Jelonek K, Wojakowska M, Pietrowska M, Polanska J, Marczak L, Miszczyk L, Składowski K (2015): Serum proteome signature of radiation response: upregulation of inflammation-related factors, and downregulation of apolipoproteins and coagulation factors in cancer patients subjected to radiotherapy – a pilot study. Int J Radiat Oncol Biol Phys. 92: 1108-15.

Wojakowska A, Chekan M, Marczak Ł, Polanski K, Lange D, Pietrowska M, Widlak P (2015): Detection of metabolites discriminating subtypes of thyroid cancer: molecular profiling of FFPE samples using the GC/MS approach. Mol Cell Endocrinol. 417: 149-57.

Wojakowska A, Chekan M, Widłak P, Pietrowska M (2015): Application of metabolomics in thyroid cancer research. Int J Endocrinol. 2015: 258763.

Wojakowska A, Marczak Ł, Jelonek K, Polanski K, Widlak P, Pietrowska M (2015): An optimized method of metabolite extraction from formalin-fixed paraffin-embedded tissue for GC/MS analysis. PLoS One 10: e0136902.

Pietrowska M, Jelonek K, Michalak M, Roś M, Rodziewicz P, Chmielewska K, Polański K, Polańska J, Gdowicz-Kłosok A, Giglok M, Suwiński R, Tarnawski R, Dziadziuszko R, Rzyman W, Widłak P (2014): Identification of serum proteome components associated with progression of non-small cell lung cancer. Acta Biochim Pol. 61: 325-31.

Jelonek K, Pietrowska M, Roś M, Zagdański A, Suchwałko A, Polańska J, Marczyk M, Rutkowski T, Składowski K, Clench MR, Widlak P (2014): Radiation-induced changes in serum lipidome of head and neck cancer patients. Int J Mol Sci. 15: 6609-24.

Widłak P, Pietrowska M, Polańska J, Rutkowski T, Jelonek K, Kalinowska-Herok M, Gdowicz-Kłosok A, Wygoda A, Tarnawski R, Składowski K (2013): Radiotherapy-related changes in serum proteome patterns of head and neck cancer patients; the effect of low and medium doses of radiation delivered to large volumes of normal tissue. J Transl Med. 11: 299.

Kalinowska-Herok M, Pietrowska M, Walaszczyk A, Widlak P (2013): MALDI Imaging Mass Spectrometry – A Novel Approach in Biomedical Research of Tissues. Curr Proteomics 10(2): 76-82.

Jelonek K, Roś M, Pietrowska M, Widłak P (2013): Cancer biomarkers and mass spectrometry-based analyses of phospholipids in body fluids. Clin Lipidol. 8: 137-50.

Pietrowska M, Widłak P (2012): MALDI-MS-based profiling of serum proteome: detection of changes related to progression of cancer and response to anticancer treatment. Int J Proteomics 2012: 926427.

Pietrowska M, Polańska J, Suwiński R, Wideł M, Rutkowski T, Marczyk M, Marczak L, Polański A, Widłak P (2012): Comparison of peptide cancer signatures identified by mass spectrometry in serum of patients with head and neck, lung, and colorectal cancers. Int J Oncol. 40: 48-156.

Pietrowska M, Polańska J, Walaszczyk A, Wygoda A, Rutkowski T, Składowski K, Marczak L, Stobiecki M, Marczyk M, Polański A, Widłak P (2011): Association between plasma proteome profiles analysed by mass spectrometry, a lymphocyte-based DNA-break repair assay and radiotherapy-induced acute mucosal reaction in head and neck cancer patients. Int J Radiat Biol. 87: 711-9.

Widłak P, Pietrowska M, Wojtkiewicz K, Rutkowski T, Wygoda A, Marczak L, Marczyk M, Polańska J, Walaszczyk A, Domińczyk I, Składowski K, Stobiecki M, Polański A (2011): Radiation-related changes in serum proteome profiles detected by mass spectrometry in blood of patients treated with radiotherapy due to larynx cancer. J Radiat Res. 52: 575-81.

Pietrowska M, Polańska J, Marczak Ł, Behrendt K, Nowicka E, Stobiecki M, Polański A, Tarnawski R, Widłak P (2010): Mass spectrometry-based analysis of therapy-related changes in serum proteome patterns of patients with early stage breast cancer. J Translat Med. 8: e66.

Pietrowska M, Marczak Ł, Polańska J, Nowicka E, Behrendt K, Tarnawski R, Stobiecki M, Polański A, Widłak P (2010): Optimizing of MALDI-ToF-based low-molecular-weight serum proteome pattern analysis in detection of breast cancer patients; the effect of albumin removal on classification performance. Neoplasma 57: 537-44.


Pietrowska M, Marczak Ł, Polańska J, Behrendt K, Nowicka E, Walaszczyk A, Chmura A, Deja R, Stobiecki M, Polański A, Tarnawski R, Widłak P (2009): Mass spectrometry-based serum proteome pattern analysis in molecular diagnostics of early stage breast cancer. J Translat Med. 7: e60.


Wilk A, Gawin M, Frątczak K, Widłak P, Fujarewicz K (2020): On Stability of FeatureSelectionBased on MALDI Mass SpectrometryImaging Data and SimulatedBiopsy. In: Korbicz J., Maniewski R., Patan K., Kowal M. (eds) CurrentTrends in Biomedical Engineering and Bioimages Analysis. PCBEE 2019. Advances in Intelligent Systems and Computing, vol 1033; Springer, Cham 2020.

Polanski A, Marczyk M, Pietrowska M, Widlak P, Polanska J (2018): Initializing the EM algorithm for univariateGaussian, multi-component, heteroscedasticmixturemodels by dynamicprogrammingpartitions. Int J Comput Methods 15: e1850012.

Jelonek K, Pietrowska M, Widłak P (2016): Główne zmiany proteomu i metabolomu surowicy obserwowane w krwi osób poddanych radioterapii dotyczą wskaźników stanu zapalnego i odpowiedzi immunologicznej odzwierciedlających systemową toksyczność promieniowania jonizującego. Onkologia I Radioterapia 3, 24-29

Kalinowska-Herok M, Roś M, Widłak P. (2013): Molekularne marginesy guza nowotworowego. Nowotwory – J. Oncol. 63: 28-34.

Hanus J, Jelonek K, Pietrowska P (2011): Wykorzystanie spektrometrii mas do analizy modyfikacji nukleotydów i adduktów DNA. Wiadomości Chemiczne 65: 191-205.

Chmura A, Deja R, Mrochem-Kwarciak J, Masłyk B, Polańska J, Pietrowska M, Ponge L, Behrendt K, Nowicka E, Tarnawski R, Widłak P (2011): Analiza zmian stężenia osteopontyny w osoczu krwi chorych leczonych z powodu nisko zaawansowanego raka piersi. Onkologia Info 8: 4-10.

Wojtkiewicz K, Marczyk M, Polańska J, Polański A, Marczak Ł, Stobiecki M, Pietrowska M, Domińczyk I, Behrendt K, Nowicka E, Tarnawski R, Widłak P (2010): Wykorzystanie spektrometrii mas do analizy proteomu surowicy chorych na raka piersi. Onkologia Info 7: 40-7.

Pietrowska M (2009): Tumor markers studied with proteomic methods in blood and serum plasma. Biotechnologia 2009(2): 39-53.

Plechawska M, Polańska J, Polański A, Pietrowska M, Tarnawski R , Widłak P, Stobiecki M, Marczak Ł (2009): Analyze of MALDI-TOF Proteomic Spectra with Usage of Mixture of Gaussian Distributions. (in:) Advances in Soft Computing , 2009, Volume 59 , Man-Machine Interactions (eds. KA Cyran, S Kozielski , JF Peters, U Stanczyk, A Wakulicz-Deja) , pp. 113-120

Polańska J, Widłak P, Rzeszowska-Wolny J, Kimmel M, Polański A (2007): Gaussian mixture decomposition of time-course DNA microarray data. w: Mathematical Modeling of Biological Systems: Cellular Biophysics, Regulatory Network, Development, Biomedicine, and Data Analysis, volume I, ed. A. Deutsch; Birkhäuser& Springer, Cambridge, str.: 351-359.

Pietrowska M, Marczak Ł, Widłak P (2007): Proteomika kliniczna – wykorzystanie metod spektrometrii mas do analizy proteomu surowicy krwi w diagnostyce chorób nowotworowych. w: Na Pograniczu Chemii i Biologii, tom. XVII, str. 93-109. Wyd. UAM, Poznań.



Abramowicz A, Wojakowska A, Marczak L, Lysek-Gladysinska M, Smolarz M, Story MD, Polanska J, Widlak P, Pietrowska M (2019):Ionizing radiation affects the composition of the proteome of extracellular vesicles released by head-and-neck cancer cells in vitro. J Radiat Res. 60: 289-97.

Ludwig S, Marczak L, Sharma P, Abramowicz A, Gawin M, Widlak P, Whiteside TL, Pietrowska M (2019): Proteomes of exosomes from HPV(+) or HPV(-) head and neck cancer cells: differential enrichment in immunoregulatory proteins. OncoImmunology 8: e1593808.

Smolarz M, Pietrowska M, Matysiak N, Mielańczyk Ł, Widłak P (2019): Proteome Profiling of Exosomes Purified from a Small Amount of Human Serum: The Problem of Co-Purified Serum Components. Proteomes 7: E18.

Abramowicz A, Widłak P, Pietrowska M (2019):Different Types of Cellular Stress Affect the Proteome Composition of Small Extracellular Vesicles: A Mini Review. Proteomes 7: E23.

Zebrowska A, Skowronek A, Wojakowska A, Widlak P, Pietrowska M (2019): Metabolome of Exosomes: Focus on Vesicles Released by Cancer Cells and Present in Human Body Fluids. Int J Mol Sci. 20: E3461.

Abramowicz A, Marczak L, Wojakowska A, Zapotoczny S, Whiteside TL, Widlak P, Pietrowska M (2018): Harmonization of exosome isolation from culture supernatants for optimized proteomics analysis. PLoS One 13: e0205496.

Pietrowska M, Funk S, Gawin M, Marczak Ł, Abramowicz A, Widłak P, Whiteside T (2017): Isolation of exosomes for the purpose of protein cargo analysis with the use of mass spectrometry. Methods Mol Biol. 1654: 291-307.

Jelonek K, Widlak P, Pietrowska M (2016): The Influence of Ionizing Radiation on Exosome Composition, Secretion and Intercellular Communication. Protein Pept Lett. 23: 656-63.

Abramowicz A, Widlak P, Pietrowska M (2016): Proteomic analysis of exosomal cargo: the challenge of high purity vesicle isolation. Mol Biosyst. 12: 1407-19.

Jelonek K, Wojakowska A, Marczak L, Muer A, Tinhofer-Keilholz I, Lysek-Gladysinska M, Widlak P, Pietrowska M (2015): Ionizing radiation affects protein composition of exosomes secreted in vitro from head and neck squamous cell carcinoma. Acta Biochim Pol. 62: 265-72.


HSF1- and NF-κB-dependent signaling pathways

Janus P, Mrowiec K, Vydra N, Widłak P, Toma-Jonik A, Korfanty J, Smolarczyk R, Widłak W (2020): PHLDA1 does not contribute directly to heat shock-induced apoptosis of spermatocytes. Int J Mol Sci. 21: 267.

Szołtysek K, Janus P, Zając G, Stokowy T, Walaszczyk A, Widłak W, Wojtaś B, Gielniewski B, Cockell S, Perkins ND, Kimmel M, Widlak P (2018): RRAD, IL4I1, CDKN1A, and SERPINE1 genes are potentially co-regulated by NF-κB and p53 transcription factors in cells exposed to high doses of ionizing radiation. BMC Genomics. 19: 813.

Janus P, Szołtysek K, Zając G, Stokowy T, Walaszczyk A, Widłak W, Wojtaś B, Gielniewski B, Iwanaszko M, Braun R, Cockell S, Perkins ND, Kimmel M, Widlak P (2018): Pro-inflammatory cytokine and high doses of ionizing radiation have similar effects on the expression of NF-kappaB-dependent genes. Cell Signal. 46: 23-31.

Szoltysek K, Walaszczyk A, Janus P, Kimmel M, Widlak P (2017): Irradiation with UV-C inhibits TNF-α-dependent activation of the NF-κB pathway in a mechanism potentially mediated by reactive oxygen species. Genes Cells. 22: 45-58.

Jonak K, Kurpas M, Szoltysek K, Janus P, Abramowicz A, Puszynski K (2016): A novel mathematical model of ATM/p53/NF- κB pathways points to the importance of the DDR switch-off mechanisms. BMC Syst Biol.10: 75.

Janus P, Stokowy T, Jaksik R, Szoltysek K, Handschuh L, Podkowinski J, Widlak W, Kimmel M, Widlak P (2015): Cross talk between cytokine and hyperthermia-induced pathways: identification of different subsets of NF-κB-dependent genes regulated by TNFα and heat shock. Mol Genet Genomics 290: 1979-90.

Toma-Jonik A, Widlak W, Korfanty J, Cichon T, Smolarczyk R, Gogler-Piglowska A, Widlak P, Vydra N (2015): Active heat shock transcription factor 1 supports migration of the melanoma cells via vinculin down-regulation. Cell Signal. 27: 394-401.

Korfanty J, Stokowy T, Widlak P, Gogler-Piglowska A, Handschuh L, Podkowiński J, Vydra N, Naumowicz A, Toma-Jonik A, Widlak W (2014): Crosstalk between HSF1 and HSF2 during the heat shock response in mouse testes. Int J Biochem Cell Biol. 57: 76-83.

Janus P, Pakuła-Cis M, Kalinowska-Herok M, Kashchak N, Szołtysek K, Pigłowski W, Widłak W, Kimmel M, Widłak P (2011): NF- kappa B signaling pathway is inhibited by heat shock independently of active transcription factor HSF1 and increased levels of inducible heat shock proteins. Genes Cells 16: 1168-75.

Szołtysek K, Pietranek K, Kalinowska-Herok M, Pietrowska M, Kimmel M, Widłak P (2008): TNF a -induced activation of NF kappa B protects against UV-induced apoptosis specifically in p53-proficient cells. Acta Biochim Polon. 55: 741-8.

Widłak W, Vydra N, Malusecka E, Dudaladava V, Winiarski B, Ścieglinska D, Widłak P (2007): Heat shock transcription factor 1 down-regulates spermatocyte-specific 70 kDa heat shock protein expression prior to the induction of apoptosis in mouse testes. Genes Cells 12: 487-99.

Vydra N, Małusecka E, Jarząb M, Lisowska K, Głowala-Kosińska M, Benedykt K, Widłak P, Krawczyk Z, Widłak W (2006): Spermatocyte-specific expression of constitutively active heat shock factor 1 induces HSP70i-resistant apoptosis in male germ cells. Cell Death Differ. 13: 212-22.


Szołtysek K, Janus P, Widłak (2011): Komórkowa ścieżka sygnałowa zależna od czynnika transkrypcyjnego NF-kB i jej współzależności ze szlakami p53 i HSF1. Postępy Biologii Komórki 38: 159-75.

Widłak P, Gramatyka M, Kimmel M (2014): Crosstalk between stress-induced NF-κB, p53 and HSF1 signaling pathways – review. Preprints of the 19th World Congress of the International Federation of Automatic Control: 11518-11523.


Apoptotic nucleases and chromatin fragmentation

Hanus J, Kalinowska-Herok M, Widłak P (2010): Identification of novel putative regulators of the major apoptotic nuclease DNA Fragmentation Factor. Acta Biochim Polon. 57: 521-527.

Widłak P, Garrard WT (2009): Roles of the major apoptotic nuclease - DNA Fragmentation Factor - in biology and disease. Cell Mol Life Sci. 66: 263-74.

Kalinowska-Herok M, Widłak P (2008): High Mobility Group proteins stimulate DNA cleavage by apoptotic endonuclease DFF40/CAD due to HMG-box interactions with DNA. Acta Biochim Polon. 55: 21-6.

Hanus J, Kalinowska-Herok M, Widłak P (2008): The major apoptotic endonuclease DFF40/CAD is a deoxyribose-specific and double-strand-specific enzyme. Apoptosis 13: 377-82.

Xiao F, Widłak P, Garrard WT (2007): Engineered apoptotic nucleases for chromatin research. Nucl Acids Res. 35, e93.

Sikora E, Bielak-Żmijewska A, Magalska A, Piwocka K, Mosieniak G, Kalinowska M, Widłak P, Cymerman IA, Bujnicki JM (2006): Curcumin induces caspase-3-dependent apoptotic pathway but inhibits DNA fragmentation factor 40/caspase-activated DNase endonuclease in human Jurkat cells. Mol Cancer Ther. 5: 927-34.

Widłak P, Garrard WT (2006): The apoptotic endonuclease DFF40/CAD is inhibited by RNA, heparin and other polyanions. Apoptosis 11: 1331-7.

Widłak P, Garrard WT (2006): Unique features of the apoptotic endonuclease DFF40/CAD relative to micrococcal nuclease as a structural probe for chromatin. Biochem Cell Biol. 84: 405-410.

Widłak P, Garrard WT (2005): Discovery, regulation, and action of the major apoptotic nucleases DFF40/CAD and endonuclease G. J Cell Biochem. 94: 1078-87.

Widłak P, Kalinowska M , Parseghian MH, Lu X, Hansen JC, Garrard WT (2005): The histone H1 C-terminal domain binds to the apoptotic nuclease, DNA Fragmentation Factor (DFF40/CAD) and stimulates DNA cleavage. Biochemistry 44: 7871-8.

Kalinowska M, Garncarz W, Pietrowska M, Garrard WT, Widłak P (2005): Regulation of the human apoptotic DNase/RNase EndoG; involvement of Hsp70 and ATP. Apoptosis 10: 821-30.

Widłak P, Łanuszewska J, Cary RB, Garrard WT (2003): Subunit structures and stoichiometries of human DFF proteins before and after induction of apoptosis. J Biol Chem. 278: 26915-22.

Widłak P, Palyvoda O, Kumala S, Garrard WT (2002): Modeling apoptotic chromatin condensation in normal cell nuclei; requirement for intranuclear mobility and actin involvement. J Biol Chem. 277: 21683-90.

Widłak P, Li LY, Wang X, Garrard WT (2001): Action of recombinant human apoptotic endonuclease G on naked DNA and chromatin substrates; cooperation with exonuclease and DNaseI. J Biol Chem. 276: 48404-9.

Widłak P, Garrard WT (2001): Ionic and cofactor requirements for the activity of the apoptotic endonuclease DFF40/CAD. Mol Cell Biochem. 218: 125-30.

Widłak P, Li P, Wang X, Garrard W (2000): Cleavage preferences of the apoptotic endonuclease DFF40 (Caspase-activated DNase or Nuclease) on naked DNA and chromatin substrates. J Biol Chem. 275: 8226-32.

Widłak P (2000): DFF40/CAD hypersensitive sites are potentially involved in high molecular weight DNA fragmentation during apoptosis. Cell Mol Biol Lett. 5: 373-9.

Widłak P. (2000): The DFF40/CAD endonuclease and its role in apoptosis. Acta Biochim Polon. 47: 1037-44.


Widłak P (2000): Mechanizmy fragmentacji DNA i kondensacji chromatyny w komórkach ulegających apoptozie. Postępy Biologii Komórki 27: 583-97.


Damaged DNA-binding proteins

Widłak P, Pietrowska M, Łanuszewska J (2006): The role of chromatin proteins in DNA damage recognition and repair. Histochem Cell Biol. 125: 119-26.

Pietrowska M, Kołodziejczyk I, Widłak P (2006): Mitochondrial transcription factor A is the major protein in rodent hepatocytes that recognizes DNA lesions induced by N-acetoxy-acetylaminofluorene. Acta Biochim Polon. 53: 777-82.

Pietrowska M, Widłak P (2005): Characterization of a novel protein that specifically binds to DNA modified by N-acetoxy-acetylaminofluorene and cis -diammine-dichloro-platinum. Acta Biochim Polon. 52: 867-74.

Łanuszewska J, Widłak P (2004): The truncation of Ku86 in human lymphocytes. Cancer Lett. 205: 197-205.

Łanuszewska J, Cudak A, Rzeszowska-Wolny J, Widłak P (2000) Detection of damage-recognition proteins from human lymphocytes. Acta Biochim Polon. 47: 443-50.

Łanuszewska J, Widłak P (2000): High mobility group 1 and 2 proteins bind preferentially to DNA that contains bulky adducts induced by benzo(a)pyrene diol epoxide and N-acetoxy-acetylaminofluorene. Cancer Lett. 158: 17-25.

Pietrowska M, Łanuszewska J, Walter Z, Rzeszowska-Wolny J, Widłak P (2000): Detection and characterization of rat protein recognizing DNA damaged by N-acetoxy-acetylaminofluorene. Cell Mol Biol Lett. 5: 423-31.


Łanuszewska J, Widłak P (2005): Udział białek chromatyny w rozpoznawaniu i naprawie uszkodzeń DNA. w: Na Pograniczu Chemii i Biologii, tom. XII, str. 269-287. Wyd. UAM, Poznań.

Łanuszewska J, Widłak P (2003): Białka detektorowe rozpoznające pęknięcia nici DNA i ich udział w mechanizmach komórkowej odpowiedzi na stres. Postępy Biochemii 49: 229-38.

Widłak P (2000): Białka rozpoznające i wiążące się z uszkodzonym DNA; udział w mechanizmach naprawy DNA i kancerogenezie. Postępy Biochemii 46: 198-206.


Other Topics

DNA damage and response to damaging factors

Balázs K, Kis E, Badie C, Bogdándi EN, Candéias S, Garcia LC, Dominczyk I, Frey B, Gaipl U, Jurányi Z, Kocsis ZS, Rutten EA, Sáfrány G, Widlak P, Lumniczky K (2019):Radiotherapy-Induced Changes in the Systemic Immune and Inflammation Parameters of Head and Neck Cancer Patients. Cancers (Basel). 11: E1324.

Cruz-Garcia L, O'Brien G, Donovan E, Gothard L, Boyle S, Laval A, Testard I, Ponge L, Woźniak G, Miszczyk L, Candéias SM, Ainsbury E, Widlak P, Somaiah N, Badie C (2018): Influence of Confounding Factors on Radiation Dose Estimation Using In Vivo Validated Transcriptional Biomarkers. Health Phys. 115: 90-101.

Wieczorek A, Lysek-Gladysinska M, Walaszczyk A, Jelonek K, Smolarz M, Pietrowska M, Gabrys D, Kulik R, Widlak P (2018):Changes in activity and structure of lysosomes from liver of mouse irradiated in vivo. Int J Radiat Biol. 94: 443-453.

Walaszczyk A, Szołtysek K, Jelonek K, Polańska J, Dörr W, Haagen J, Widłak P, Gabryś D (2018): Heart irradiation reduces microvascular density and accumulation of HSPA1 in mice. Strahlenther Onkol. 194: 235-42.

Lysek-Gladysinska M, Wieczorek A, Walaszczyk A, Jelonek K, Jozwik A, Pietrowska M, Dörr W, Gabrys D, Widlak P (2018): Long-term effects of low-dose mouse liver irradiation involve ultrastructural and biochemical changes in hepatocytes that depend on lipid metabolism. Radiat Environ Biophys. 57: 123-32.

O'Brien G, Cruz-Garcia L, Majewski M, Grepl J, Abend M, Port M, Tichý A, Sirak I, Malkova A, Donovan E, Gothard L, Boyle S, Somaiah N, Ainsbury E, Ponge L, Slosarek K, Miszczyk L, Widlak P, Green E, Patel N, Kudari M, Gleeson F, Vinnikov V, Starenkiy V, Artiukh S, Vasyliev L, Zaman A, Badie C (2018): FDXR is a biomarker of radiation exposure in vivo. Sci Rep. 8: 684.

Gramatyka M, Widłak P (2015): Neither resveratrol nor metformin protects human cardiomyocytes against toxicity of epirubicin and radiation in-vitro. Medical Science 17(67):1-10.

Jelonek K, Walaszczyk A, Gabryś D, Pietrowska M, Kanthou C, Widłak P (2011): Cardiac endothelial cells isolated from mouse heart - a novel model for radiobiology. Acta Biochim Polon. 58: 397-404.

Jelonek K, Gdowicz-Kłosok A, Pietrowska P, Borkowska M, Korfanty J, Rzeszowska-Wolny J, Widłak P (2010): Association between single-nucleotide polymorphisms of selected genes involved in the response to DNA damage and risk of colon, head and neck, and breast cancers in a Polish population. J Appl Genet. 51: 343-352.

Horak S, Olejek A, Widłak P (2007): Sperm DNA adducts impair fertilization during ICSI but not during IVF. Folia Histochem Cytobiol. 45, suppl. 1: S99-S104.

Horak S, Polańska J, Widłak P (2003): High levels of bulky DNA adducts in human sperm correlate with impaired fertility. Acta Biochim Polon. 50: 197-203.

Horak S, Polańska J, Widłak P (2003): Bulky DNA adducts in human sperm: relationship with fertility, semen quality, smoking, and environmental factors. Mutat Res. 537: 53-65.

Rzeszowska-Wolny J, Polańska J, Pietrowska M, Palyvoda O, Jaworska J, Butkiewicz D, Hancock R (2005):. Influence of polymorphisms in DNA repair genes XPD, XRCC1 and MGMT on DNA damage induced by gamma radiation and its repair in lymphocytes in vitro. Radiat Res. 164: 132-140.


Walaszczyk A, Pietrowska M (2009): Kardiotoksyczność promieniowania jonizującego. w: Na Pograniczu Chemii i Biologii, tom. XXII, str. 347-369. Wyd. UAM, Poznań.

Widłak P (2002): Struktura chromatyny a powstawanie i naprawa uszkodzeń DNA. Kosmos 51: 5-12.

Widłak P (2000): The DNA damage-induced cell cycle checkpoints. J Theor Med. 2: 273-43.

Widłak P (2000): Naprawa dwuniciowych pęknięć DNA indukowanych przez promieniowanie jonizujące. Postępy Higieny I Medycyny Doświadczalnej 54: 133-48.


Chromatin and gene regulation

Korfanty J, Stokowy T, Chadalski M, Toma-Jonik A, Vydra N, Widłak P, Wojtaś B, Gielniewski B, Widlak W (2018): SPEN protein expression and interactions with chromatin in mouse testicular cells. Reproduction. 156: 195-206.

Piccinni E, Chełstowska A, Hanus J, Widłak P , Loreti S, Tata AM, Augusti-Tocco G, Bianchi MM, Negri R (2011): Direct interaction of Gas41 and Myc encoded by amplified genes in nervous system tumours. Acta Biochim Polon. 58: 529-34.

Liu Z, Widłak P, Zou Y, Xiao F, Oh M, Li S, Chang MY, Shay JW, Garrard WT (2006): A recombination silencer that specifies heterochromatin positioning and Ikaros association in the immunoglobulin k locus. Immunity 24: 405-15.

Widłak W, Widłak P (2004): MAR/SAR elements flank the rat hst70 gene transcription unit. Cell Mol Biol Lett. 9: 123-33.

Fedorov A, Lukyanov D, Rogoliński J, Widłak P, Podgornaya O, Rzeszowska-Wolny J (2004): The nuclear protein p30 interacts with a nuclear matrix attachment region from the rat genome. Cell Mol Biol Lett. 9: 153-65.

Widłak P (2004): DNA microarrays, a novel approach in studies of chromatin structure. Acta Biochim Polon. 51: 1-8.

Weil MR, Widłak P, Minna JD, Garner HR (2004): Global survey of chromatin accessibility using DNA microarrays. Genome Res. 14: 1374-81.


Widłak P, Fujarewicz K (2003): The analysis of chromatin condensation state and transcriptional activity using DNA microarrays. Journal of Medical Informatics and Technologies. 6: IP13-IP19.


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