Major Topics

Clinical Proteomics and Metabolomics

Wojakowska A, Cole LM, Chekan M, Bednarczyk K, Maksymiak M, Oczko-Wojciechowska M, Jarząb B, Clench MR, Polańska J, Pietrowska M, Widlak P (2018): Discrimination of papillary thyroid cancer from non-cancerous thyroid tissue based on lipid profiling by mass spectrometry imaging. Endokrynol Pol. 2018; 69: 2-8.

https://www.ncbi.nlm.nih.gov/pubmed/29492952

Gawin M, Wojakowska A, Pietrowska M, Marczak Ł, Chekan M, Jelonek K, Lange D, Jaksik R, Gruca A, Widłak P (2017): Proteome profiles of different types of thyroid cancers. Mol Cell Endocrinol.  [Epub ahead of print]

https://www.ncbi.nlm.nih.gov/pubmed/29183805

Pietrowska M, Diehl HC, Mrukwa G, Kalinowska-Herok M, Gawin M, Chekan M, Elm J, Drazek G, Krawczyk A, Lange D, Meyer HE, Polanska J, Henkel C, Widlak P (2017): Molecular profiles of thyroid cancer subtypes: Classification based on features of tissue revealed by mass spectrometry imaging. Biochim Biophys Acta. 1865:837-845.

https://www.ncbi.nlm.nih.gov/pubmed/27760391

Roś-Mazurczyk M, Wojakowska A, Marczak Ł, Polański K, Pietrowska M, Jelonek K, Domińczyk I, Hajduk A, Rutkowski T, Składowski K, Widłak P (2017): Ionizing radiation affects profile of serum metabolites: increased level of 3-hydroxybutyric acid in serum of cancer patients treated with radiotherapy. Acta Biochim Pol. 64:189-193.

https://www.ncbi.nlm.nih.gov/pubmed/27815965

Jelonek K, Pietrowska M, Widlak P (2017): Systemic effects of ionizing radiation at the proteome and metabolome levels in the blood of cancer patients treated with radiotherapy: the influence of inflammation and radiation toxicity. Int J Radiat Biol. 93:683-696.

https://www.ncbi.nlm.nih.gov/pubmed/28281355

Roś-Mazurczyk M, Jelonek K, Marczyk M, Binczyk F, Pietrowska M, Polanska J, Dziadziuszko R, Jassem J, Rzyman W, Widlak P (2017): Serum lipid profile discriminates patients with early lung cancer from healthy controls. Lung Cancer. 112:69-74.

https://www.ncbi.nlm.nih.gov/pubmed/29191603

Roś-Mazurczyk M, Wojakowska A, Marczak Ł, Polański K, Pietrowska M, Polanska J, Dziadziuszko R, Jassem J, Rzyman W, Widlak P (2017): Panel of serum metabolites discriminates cancer patients and healthy participants of lung cancer screening - a pilot study. Acta Biochim Pol. 64:513-518.

https://www.ncbi.nlm.nih.gov/pubmed/28803255

Walaszczyk A, Pietrowska M, Wojakowska A, Abramowicz A, Chmura A, Masłyk B, Rodziewicz P, Polańska J, Behrendt K, Nowicka E, Tarnawski R, Widłak P (2017) Therapy-related changes in serum proteome patterns of early stage breast cancer patients with different outcome. Protein Pept Lett. 24:37-45.

https://www.ncbi.nlm.nih.gov/pubmed/27894246

Roś-Mazurczyk M, Jelonek K, Pietrowska M, Zagdański A, Suchwałko A, Jastrząb T, Polańska J, Chawińska E, Majewski W, Domińczyk I, Rutkowski T, Miszczyk L, Składowski K, Widłak P (2016): Radiotherapy-induced changes in serum lipid profile of patients with prostate or head and neck cancer. J J Rad Oncol. 2016, 3(2): 030.

http://radiationoncology.jacobspublishers.com/images/Rad_Onco/J_J_Rad_Oncol_3_2_030.pdf

Widlak P, Pietrowska M, Polanska J, Marczyk M, Ros-Mazurczyk M, Dziadziuszko R, Jassem J, Rzyman W (2016): Serum mass profile signature as a biomarker of early lung cancer. Lung Cancer 99:46-52.

https://www.ncbi.nlm.nih.gov/pubmed/27565913

Pietrowska M, Gawin M, Polańska J, Widłak P (2016): Tissue fixed with formalin and processed without paraffin embedding is suitable for imaging of both peptides and lipids by MALDI-IMS. Proteomics 16:1670-1677.

https://www.ncbi.nlm.nih.gov/pubmed/27001204

Widlak P, Mrukwa G, Kalinowska M, Pietrowska M, Chekan M, Wierzgon J, Gawin M, Drazek G, Polanska J (2016): Detection of molecular signatures of oral squamous cell carcinoma and normal epithelium - application of a novel methodology for unsupervised segmentation of imaging mass spectrometry data. Proteomics 16:1613-1621.

https://www.ncbi.nlm.nih.gov/pubmed/27168173

Szymańska K, Pietrowska M, Kocurek J, Maresz K, Koreniuk A, Mrowiec-Białoń J, Widłak P, Magner E, Jarzębski A (2016): Low back-pressure hierarchically structured multichannel microfluidic bioreactors for rapid protein digestion - proof of concept. Chem Eng J  287: 148-54.

http://dx.doi.org/10.1016/j.cej.2015.10.120

Gadher SJ, Marczak Ł, Łuczak M, Stobiecki M, Widlak P, Kovarova H (2016): Proteomic landscape in Central and Eastern Europe: the 9th Central and Eastern European Proteomic Conference, Poznań, Poland. Expert Rev Proteomics 13: 5-7. 

http://www.ncbi.nlm.nih.gov/pubmed/26558656

Liśkiewicz AD, Kasprowska D, Wojakowska A, Polański K, Lewin-Kowalik J, Kotulska K, Jędrzejowska-Szypułka H (2016) Long-term high fat ketogenic diet promotes renal tumor growth in a rat model of tuberous sclerosis. Sci Rep. 6: 21807.

https://www.ncbi.nlm.nih.gov/pubmed/26892894

Tóth E, Vékey K, Ozohanics O, Jekő A, Dominczyk I, Widlak P, Drahos L (2016): Changes of protein glycosylation in the course of radiotherapy. J Pharm Biomed Anal 118: 380-6.

http://www.ncbi.nlm.nih.gov/pubmed/26609677

Abramowicz A, Wojakowska A, Gdowicz-Klosok A, Polanska J, Rodziewicz P, Polanowski P, Namysl-Kaletka A, Pietrowska M, Wydmanski J, Widlak P (2015): Identification of serum proteome signatures of locally advanced and metastatic gastric cancer: a pilot study. J Transl Med 13: e304.

http://www.ncbi.nlm.nih.gov/pubmed/26376850

Pietrowska M, Jelonek K, Polanska J, Wojakowska A, Marczak L, Chawinska E, Chmura A, Majewski W, Miszczyk L, Widlak P (2015): Partial-body irradiation in patients with prostate cancer treated with IMRT has little effect on the composition of serum proteome. Proteomes 3: 117-31.

http://www.mdpi.com/2227-7382/3/3/117 

Polanski A, Marczyk M, Pietrowska M, Widlak P, Polanska J (2015): Signal partitioning algorithm for highly efficient Gaussian Mixture Modeling in mass spectrometry. PLoS One 10: e0134256.

http://www.ncbi.nlm.nih.gov/pubmed/26230717

Widłak P, Jelonek K, Wojakowska M, Pietrowska M, Polanska J, Marczak L, Miszczyk L, Składowski K (2015) Serum proteome signature of radiation response: upregulation of inflammation-related factors, and downregulation of apolipoproteins and coagulation factors in cancer patients subjected to radiotherapy – a pilot study. Int J Radiat Oncol Biol Phys 92: 1108-15.

http://www.ncbi.nlm.nih.gov/pubmed/26031365

Wojakowska A, Chekan M, Marczak Ł, Polanski K, Lange D, Pietrowska M, Widlak P (2015) Detection of metabolites discriminating subtypes of thyroid cancer: molecular profiling of FFPE samples using the GC/MS approach. Mol Cell Endocrinol 417: 149-57.

http://www.ncbi.nlm.nih.gov/pubmed/26415588

Wojakowska A, Chekan M, Widłak P, Pietrowska M (2015) Application of metabolomics in thyroid cancer research. Int J Endocrinol 2015:258763.

http://www.ncbi.nlm.nih.gov/pubmed/25972898

Wojakowska A, Marczak Ł, Jelonek K, Polanski K, Widlak P, Pietrowska M (2015): An optimized method of metabolite extraction from formalin-fixed paraffin-embedded tissue for GC/MS analysis. PLoS One 10:e0136902.

http://www.ncbi.nlm.nih.gov/pubmed/26348873

Pietrowska M, Jelonek K, Michalak M, Roś M, Rodziewicz P, Chmielewska K, Polański K, Polańska J, Gdowicz-Kłosok A, Giglok M, Suwiński R, Tarnawski R, Dziadziuszko R, Rzyman W, Widłak P (2014): Identification of serum proteome components associated with progression of non-small cell lung cancer. Acta Biochim. Pol. 61: 325-331.

http://www.ncbi.nlm.nih.gov/pubmed/24872961

Jelonek K, Pietrowska M, Roś M, Zagdański A, Suchwałko A, Polańska J, Marczyk M, Rutkowski T, Składowski K, Clench MR, Widlak P (2014): Radiation-induced changes in serum lipidome of head and neck cancer patients. Int. J. Mol. Sci. 15: 6609-6624.

http://www.ncbi.nlm.nih.gov/pubmed/24747595

Widłak P, Pietrowska M, Polańska J, Rutkowski T, Jelonek K, Kalinowska-Herok M, Gdowicz-Kłosok A, Wygoda A, Tarnawski R, Składowski K (2013): Radiotherapy-related changes in serum proteome patterns of head and neck cancer patients; the effect of low and medium doses of radiation delivered to large volumes of normal tissue. J. Transl. Med. 11: 299.

http://www.ncbi.nlm.nih.gov/pubmed/24304975

Kalinowska-Herok M, Pietrowska M, Walaszczyk A, Widlak P (2013): MALDI Imaging Mass Spectrometry – A Novel Approach in Biomedical Research of Tissues. Curr. Proteomics 10(2): 76-82.

http://www.ingentaconnect.com/content/ben/cp/2013/00000010/00000002/art00002

Jelonek K, Roś M, Pietrowska M, Widłak P (2013): Cancer biomarkers and mass spectrometry-based analyses of phospholipids in body fluids. Clin. Lipidol. 8: 137-150.

http://www.futuremedicine.com/doi/abs/10.2217/clp.12.79

Pietrowska M, Widłak P (2012) MALDI-MS-based profiling of serum proteome: detection of changes related to progression of cancer and response to anticancer treatment. Int. J. Proteomics 2012:926427.

http://www.ncbi.nlm.nih.gov/pubmed/22900176

Pietrowska M, Polańska J, Suwiński R, Wideł M, Rutkowski T, Marczyk M, Marczak L, Polański A, Widłak P (2012): Comparison of peptide cancer signatures identified by mass spectrometry in serum of patients with head and neck, lung, and colorectal cancers. Int. J. Oncol.40: 148-156.

http://www.ncbi.nlm.nih.gov/pubmed/21894432

Pietrowska M, Polańska J, Walaszczyk A, Wygoda A, Rutkowski T, Składowski K, Marczak L, Stobiecki M, Marczyk M, Polański A, Widłak P (2011): Association between plasma proteome profiles analysed by mass spectrometry, a lymphocyte-based DNA-break repair assay and radiotherapy-induced acute mucosal reaction in head and neck cancer patients. Int. J. Radiat. Biol. 87: 711-719.

http://www.ncbi.nlm.nih.gov/pubmed/21351848

Widłak P, Pietrowska M, Wojtkiewicz K, Rutkowski T, Wygoda A, Marczak L, Marczyk M, Polańska J, Walaszczyk A, Domińczyk I, Składowski K, Stobiecki M, Polański A (2011): Radiation-related changes in serum proteome profiles detected by mass spectrometry in blood of patients treated with radiotherapy due to larynx cancer. J. Radiat. Res. 52: 575-581.

http://www.ncbi.nlm.nih.gov/pubmed/21768750

Pietrowska M, Polańska J, Marczak Ł, Behrendt K, Nowicka E, Stobiecki M, Polański A, Tarnawski R, Widłak P (2010): Mass spectrometry-based analysis of therapy-related changes in serum proteome patterns of patients with early stage breast cancer. J. Translat. Med. 8: e66.

http://www.ncbi.nlm.nih.gov/pubmed/20618994

Pietrowska M, Marczak Ł, Polańska J, Nowicka E, Behrendt K, Tarnawski R, Stobiecki M, Polański A, Widłak P (2010): Optimizing of MALDI-ToF-based low-molecular-weight serum proteome pattern analysis in detection of breast cancer patients; the effect of albumin removal on classification performance. Neoplasma 57: 537-544.

http://www.ncbi.nlm.nih.gov/pubmed/20845992

Pietrowska M, Marczak Ł, Polańska J, Behrendt K, Nowicka E, Walaszczyk A, Chmura A, Deja R, Stobiecki M, Polański A, Tarnawski R, Widłak P (2009): Mass spectrometry-based serum proteome pattern analysis in molecular diagnostics of early stage breast cancer. J. Translat. Med. 7: e60.

http://www.ncbi.nlm.nih.gov/pubmed/19594898

also:

Jelonek K, Pietrowska M, Widłak P (2016) Główne zmiany proteomu i metabolomu surowicy obserwowane w krwi osób poddanych radioterapii dotyczą wskaźników stanu zapalnego i odpowiedzi immunologicznej odzwierciedlających systemową toksyczność promieniowania jonizującego. Onkologia I Radioterapia 3, 24-29

Kalinowska-Herok M, Roś M, Widłak P. (2013): Molekularne marginesy guza nowotworowego. Nowotwory – J. Oncol. 63: 28-34.

Hanus J, Jelonek K, Pietrowska P (2011): Wykorzystanie spektrometrii mas do analizy modyfikacji nukleotydów i adduktów DNA.Wiadomości Chemiczne 65: 191-205.

Chmura A, Deja R, Mrochem-Kwarciak J, Masłyk B, Polańska J, Pietrowska M, Ponge L, Behrendt K, Nowicka E, Tarnawski R, Widłak P (2011): Analiza zmian stężenia osteopontyny w osoczu krwi chorych leczonych z powodu nisko zaawansowanego raka piersi. Onkologia Info 8: 4-10.

Wojtkiewicz K, Marczyk M, Polańska J, Polański A, Marczak Ł, Stobiecki M, Pietrowska M, Domińczyk I, Behrendt K, Nowicka E, Tarnawski R, Widłak P (2010): Wykorzystanie spektrometrii mas do analizy proteomu surowicy chorych na raka piersi. Onkologia Info 7: 40-47.

Pietrowska M (2009): Tumor markers studied with proteomic methods in blood and serum plasma. Biotechnologia 2009(2): 39-53.

Plechawska M, Polańska J, Polański A, Pietrowska M, Tarnawski R , Widłak P, Stobiecki M, Marczak Ł (2009): Analyze of MALDI-TOF Proteomic Spectra with Usage of Mixture of Gaussian Distributions. (in:) Advances in Soft Computing , 2009, Volume 59 , Man-Machine Interactions (eds. KA Cyran, S Kozielski , JF Peters, U Stanczyk, A Wakulicz-Deja) , pp. 113-120

Polańska J, Widłak P, Rzeszowska-Wolny J, Kimmel M, Polański A (2007): Gaussian mixture decomposition of time-course DNA microarray data. w: Mathematical Modeling of Biological Systems: Cellular Biophysics, Regulatory Network, Development, Biomedicine, and Data Analysis, volume I, ed. A. Deutsch; Birkhäuser & Springer, Cambridge, str.: 351-359.

Pietrowska M, Marczak Ł, Widłak P (2007): Proteomika kliniczna – wykorzystanie metod spektrometrii mas do analizy proteomu surowicy krwi w diagnostyce chorób nowotworowych. w: Na Pograniczu Chemii i Biologii, tom. XVII, str. 93-109. Wyd. UAM, Poznań.

 

Exosomes

Pietrowska M, Funk S, Gawin M, Marczak Ł, Abramowicz A, Widłak P, Whiteside T (2017): Isolation of exosomes for the purpose of protein cargo analysis with the use of mass spectrometry. Methods Mol Biol. 1654:291-307.

https://www.ncbi.nlm.nih.gov/pubmed/28986800

Jelonek K, Widlak P, Pietrowska M (2016): The Influence of Ionizing Radiation on Exosome Composition, Secretion and Intercellular Communication. Protein Pept Lett. 23: 656-663.

https://www.ncbi.nlm.nih.gov/pubmed/27117741

Abramowicz A, Widlak P, Pietrowska M (2016): Proteomic analysis of exosomal cargo: the challenge of high purity vesicle isolation. Mol Biosyst. 12:1407-1419.

https://www.ncbi.nlm.nih.gov/pubmed/27030573

Jelonek K, Wojakowska A, Marczak L, Muer A, Tinhofer-Keilholz I, Lysek-Gladysinska M, Widlak P, Pietrowska M (2015): Ionizing radiation affects protein composition of exosomes secreted in vitro from head and neck squamous cell carcinoma. Acta Biochim Pol 62: 265-272.

http://www.ncbi.nlm.nih.gov/pubmed/26098714

 

HSF1- and NF-κB-dependent signaling pathways[PW3] 

Janus P, Szołtysek K, Zając G, Stokowy T, Walaszczyk A, Widłak W, Wojtaś B, Gielniewski B, Iwanaszko M, Braun R, Cockell S, Perkins ND, Kimmel M, Widlak P (2018): Pro-inflammatory cytokine and high doses of ionizing radiation have similar effects on the expression of NF-kappaB-dependent genes. Cell Signal. 2018; 46: 23-31.

https://www.ncbi.nlm.nih.gov/pubmed/29476964

Szoltysek K, Walaszczyk A, Janus P, Kimmel M, Widlak P (2017): Irradiation with UV-C inhibits TNF-α-dependent activation of the NF-κB pathway in a mechanism potentially mediated by reactive oxygen species. Genes Cells. 22: 45-58.

https://www.ncbi.nlm.nih.gov/pubmed/27976481

Jonak K, Kurpas M, Szoltysek K, Janus P, Abramowicz A, Puszynski K (2016): A novel mathematical model of ATM/p53/NF- κB pathways points to the importance of the DDR switch-off mechanisms. BMC Syst Biol. 10: 75.

https://www.ncbi.nlm.nih.gov/pubmed/27526774

Janus P, Stokowy T, Jaksik R, Szoltysek K, Handschuh L, Podkowinski J, Widlak W, Kimmel M, Widlak P (2015): Cross talk between cytokine and hyperthermia-induced pathways: identification of different subsets of NF-κB-dependent genes regulated by TNFα and heat shock. Mol Genet Genomics 290:1979-90.

http://www.ncbi.nlm.nih.gov/pubmed/25944781

Toma-Jonik A, Widlak W, Korfanty J, Cichon T, Smolarczyk R, Gogler-Piglowska A, Widlak P, Vydra N (2015): Active heat shock transcription factor 1 supports migration of the melanoma cells via vinculin down-regulation. Cell Signal 27:394-401.

http://www.ncbi.nlm.nih.gov/pubmed/25435429

Korfanty J, Stokowy T, Widlak P, Gogler-Piglowska A, Handschuh L, Podkowiński J, Vydra N, Naumowicz A, Toma-Jonik A, Widlak W (2014): Crosstalk between HSF1 and HSF2 during the heat shock response in mouse testes. Int J Biochem Cell Biol. 57: 76-83.

http://www.ncbi.nlm.nih.gov/pubmed/25450459

Janus P, Pakuła-Cis M, Kalinowska-Herok M, Kashchak N, Szołtysek K, Pigłowski W, Widłak W, Kimmel M, Widłak P (2011): NF- kappa B signaling pathway is inhibited by heat shock independently of active transcription factor HSF1 and increased levels of inducible heat shock proteins. Genes Cells 16: 1168-1175.

http://www.ncbi.nlm.nih.gov/pubmed/22077664

Szołtysek K, Pietranek K, Kalinowska-Herok M, Pietrowska M, Kimmel M, Widłak P (2008): TNF a -induced activation of NF kappa B protects against UV-induced apoptosis specifically in p53-proficient cells. Acta Biochim. Polon 55: 741-748.

http://www.ncbi.nlm.nih.gov/pubmed/19023456

Widłak W, Vydra N, Malusecka E, Dudaladava V, Winiarski B, Ścieglinska D, Widłak P (2007): Heat shock transcription factor 1 down-regulates spermatocyte-specific 70 kDa heat shock protein expression prior to the induction of apoptosis in mouse testes. Genes Cells12: 487-499.

http://www.ncbi.nlm.nih.gov/pubmed/17397396

Vydra N, Małusecka E, Jarząb M, Lisowska K, Głowala-Kosińska M, Benedykt K, Widłak P, Krawczyk Z, Widłak W (2006): Spermatocyte-specific expression of constitutively active heat shock factor 1 induces HSP70i-resistant apoptosis in male germ cells. Cell Death Differ .13: 212-222.

http://www.ncbi.nlm.nih.gov/pubmed/16151457

also:

Szołtysek K, Janus P, Widłak (2011): Komórkowa ścieżka sygnałowa zależna od czynnika transkrypcyjnego NF-kB i jej współzależności ze szlakami p53 i HSF1. Postępy Biologii Komórki 38: 159-175.

Widłak P, Gramatyka M, Kimmel M (2014): Crosstalk between stress-induced NF-κB, p53 and HSF1 signaling pathways – review. Preprints of the 19th World Congress of the International Federation of Automatic Control: 11518-11523.

 

Apoptotic nucleases and chromatin fragmentation

Hanus J, Kalinowska-Herok M, Widłak P (2010): Identification of novel putative regulators of the major apoptotic nuclease DNA Fragmentation Factor. Acta Biochim. Polon57: 521-527.

http://www.ncbi.nlm.nih.gov/pubmed/21152448

Widłak P, Garrard WT (2009): Roles of the major apoptotic nuclease - DNA Fragmentation Factor - in biology and disease. Cell. Mol. Life Sci 66: 263-274.

http://www.ncbi.nlm.nih.gov/pubmed/18810317

Kalinowska-Herok M, Widłak P (2008): High Mobility Group proteins stimulate DNA cleavage by apoptotic endonuclease DFF40/CAD due to HMG-box interactions with DNA. Acta Biochim. Polon 55: 21-26.

http://www.ncbi.nlm.nih.gov/pubmed/18239742

Hanus J, Kalinowska-Herok M, Widłak P (2008): The major apoptotic endonuclease DFF40/CAD is a deoxyribose-specific and double-strand-specific enzyme. Apoptosis 13: 377-382.

http://www.ncbi.nlm.nih.gov/pubmed/18283539

Xiao F, Widłak P, Garrard WT (2007): Engineered apoptotic nucleases for chromatin research. Nucl. Acids Res 35, e93.

http://www.ncbi.nlm.nih.gov/pubmed/17626049

Sikora E, Bielak-Żmijewska A, Magalska A, Piwocka K, Mosieniak G, Kalinowska M, Widłak P, Cymerman IA, Bujnicki JM (2006): Curcumin induces caspase-3-dependent apoptotic pathway but inhibits DNA fragmentation factor 40/caspase-activated DNase endonuclease in human Jurkat cells . Mol. Cancer Ther. 5: 927-934.

http://www.ncbi.nlm.nih.gov/pubmed/16648563

Widłak P, Garrard WT (2006): The apoptotic endonuclease DFF40/CAD is inhibited by RNA, heparin and other polyanions. Apoptosis 11: 1331-1337.

http://www.ncbi.nlm.nih.gov/pubmed/16699957

Widłak P, Garrard WT (2006): Unique features of the apoptotic endonuclease DFF40/CAD relative to micrococcal nuclease as a structural probe for chromatin. Biochem. Cell Biol 84: 405-410.

http://www.ncbi.nlm.nih.gov/pubmed/16936813

Widłak P, Garrard WT (2005): Discovery, regulation, and action of the major apoptotic nucleases DFF40/CAD and endonuclease G. J. Cell. Biochem 94: 1078-1087.

http://www.ncbi.nlm.nih.gov/pubmed/15723341

Widłak P, Kalinowska M , Parseghian MH, Lu X, Hansen JC, Garrard WT (2005): The histone H1 C-terminal domain binds to the apoptotic nuclease, DNA Fragmentation Factor (DFF40/CAD) and stimulates DNA cleavage. Biochemistry,44: 7871-7878.

http://www.ncbi.nlm.nih.gov/pubmed/15910001

Kalinowska M, Garncarz W, Pietrowska M, Garrard WT, Widłak P (2005): Regulation of the human apoptotic DNase/RNase EndoG; involvement of Hsp70 and ATP. Apoptosis 10: 821-830.

http://www.ncbi.nlm.nih.gov/pubmed/16133872

Widłak P, Łanuszewska. J, Cary RB, Garrard WT (2003): Subunit structures and stoichiometries of human DFF proteins before and after induction of apoptosis. J. Biol. Chem. 278: 26915-26922.

http://www.ncbi.nlm.nih.gov/pubmed/12748178

Widłak P, Palyvoda O, Kumala S, Garrard WT (2002): Modeling apoptotic chromatin condensation in normal cell nuclei; requirement for intranuclear mobility and actin involvement. J. Biol. Chem 277: 21683-21690.

http://www.ncbi.nlm.nih.gov/pubmed/11927586

Widłak P, Li LY, Wang X, Garrard WT (2001): Action of recombinant human apoptotic endonuclease G on naked DNA and chromatin substrates; cooperation with exonuclease and DNaseI. J. Biol. Chem 276: 48404–48409.

http://www.ncbi.nlm.nih.gov/pubmed/11606588

Widłak P, Garrard WT (2001): Ionic and cofactor requirements for the activity of the apoptotic endonuclease DFF40/CAD. Mol. Cell. Biochem 218: 125-130.

http://www.ncbi.nlm.nih.gov/pubmed/11330826

Widłak P, Li P, Wang X, Garrard W (2000): Cleavage preferences of the apoptotic endonuclease DFF40 (Caspase-activated DNase or Nuclease) on naked DNA and chromatin substrates. J. Biol. Chem. 275: 8226-8232.

http://www.ncbi.nlm.nih.gov/pubmed/10713148

Widłak P (2000): DFF40/CAD hypersensitive sites are potentially involved in high molecular weight DNA fragmentation during apoptosis.Cell. Mol. Biol. Lett 5: 373-379.

http://www.cmbl.org.pl/pdf/Vol5_p373-379.pdf

Widłak P. (2000): The DFF40/CAD endonuclease and its role in apoptosis. Acta Biochim. Polon 47: 1037-1044.

http://www.ncbi.nlm.nih.gov/pubmed/11996094

also:

Widłak P (2000): Mechanizmy fragmentacji DNA i kondensacji chromatyny w komórkach ulegających apoptozie. Postępy Biologii Komórki27: 583-597.

 

Damaged DNA-binding proteins

Widłak P, Pietrowska M, Łanuszewska J (2006): The role of chromatin proteins in DNA damage recognition and repair. Histochem Cell Biol. 125: 119-126.

http://www.ncbi.nlm.nih.gov/pubmed/16163486

Pietrowska M, Kołodziejczyk I, Widłak P (2006): Mitochondrial transcription factor A is the major protein in rodent hepatocytes that recognizes DNA lesions induced by N-acetoxy-acetylaminofluorene. Acta Biochim. Polon 53: 777-782.

http://www.ncbi.nlm.nih.gov/pubmed/17143337

Pietrowska M, Widłak P (2005): Characterization of a novel protein that specifically binds to DNA modified by N-acetoxy-acetylaminofluorene and cis -diammine-dichloro-platinum. Acta Biochim. Polon ., 52: 867-874.

http://www.ncbi.nlm.nih.gov/pubmed/15940347

Łanuszewska J, Widłak P (2004): The truncation of Ku86 in human lymphocytes. Cancer Lett 205: 197-205.

http://www.ncbi.nlm.nih.gov/pubmed/15036652

Łanuszewska J, Cudak A, Rzeszowska-Wolny J, Widłak P (2000) Detection of damage-recognition proteins from human lymphocytes.Acta Biochim. Polon 47: 443-450.

http://www.ncbi.nlm.nih.gov/pubmed/11051209

Łanuszewska J, Widłak P (2000): High mobility group 1 and 2 proteins bind preferentially to DNA that contains bulky adducts induced by benzo(a)pyrene diol epoxide and N-acetoxy-acetylaminofluorene. Cancer Lett 158: 17-25.

http://www.ncbi.nlm.nih.gov/pubmed/10940504

Pietrowska M, Łanuszewska J, Walter Z, Rzeszowska-Wolny J, Widłak P (2000): Detection and characterization of rat protein recognizing DNA damaged by N-acetoxy-acetylaminofluorene. Cell. Mol. Biol. Lett 5: 423-431.

http://www.cmbl.org.pl/pdf/Vol5_p423-431.pdf

also:

Łanuszewska J, Widłak P (2005): Udział białek chromatyny w rozpoznawaniu i naprawie uszkodzeń DNA. w: Na Pograniczu Chemii i Biologii, tom. XII, str. 269-287. Wyd. UAM, Poznań.

Łanuszewska J, Widłak P (2003): Białka detektorowe rozpoznające pęknięcia nici DNA i ich udział w mechanizmach komórkowej odpowiedzi na stres. Postępy Biochemii 49: 229-238.

Widłak P (2000): Białka rozpoznające i wiążące się z uszkodzonym DNA; udział w mechanizmach naprawy DNA i kancerogenezie. Postępy Biochemii 46: 198-206.

 

Other Topics

DNA damage and response to damaging factors

Walaszczyk A, Szołtysek K, Jelonek K, Polańska J, Dörr W, Haagen J, Widłak P, Gabryś D (2018): Heart irradiation reduces microvascular density and accumulation of HSPA1 in mice. Strahlenther Onkol. 2018; 194: 235-242.

https://www.ncbi.nlm.nih.gov/pubmed/29063166

Lysek-Gladysinska M, Wieczorek A, Walaszczyk A, Jelonek K, Jozwik A, Pietrowska M, Dörr W, Gabrys D, Widlak P (2018): Long-term effects of low-dose mouse liver irradiation involve ultrastructural and biochemical changes in hepatocytes that depend on lipid metabolism. Radiat Environ Biophys. 2018 [Epub ahead of print]

https://www.ncbi.nlm.nih.gov/pubmed/29470638

O'Brien G, Cruz-Garcia L, Majewski M, Grepl J, Abend M, Port M, Tichý A, Sirak I, Malkova A, Donovan E, Gothard L, Boyle S, Somaiah N, Ainsbury E, Ponge L, Slosarek K, Miszczyk L, Widlak P, Green E, Patel N, Kudari M, Gleeson F, Vinnikov V, Starenkiy V, Artiukh S, Vasyliev L, Zaman A, Badie C (2018): FDXR is a biomarker of radiation exposure in vivo. Sci Rep. 2018; 8: 684.

https://www.ncbi.nlm.nih.gov/pubmed/29330481

Gramatyka M, Widłak P (2015): Neither resveratrol nor metformin protects human cardiomyocytes against toxicity of epirubicin and radiation in-vitro. Medical Science, 2015,17(67):1-10.

http://discoveryjournals.com/medicalscience/current_issue/v17/n67/A1.pdf

Jelonek K, Walaszczyk A, Gabryś D, Pietrowska M, Kanthou C, Widłak P (2011): Cardiac endothelial cells isolated from mouse heart - a novel model for radiobiology. Acta Biochim. Polon 58 : 397-404.

http://www.ncbi.nlm.nih.gov/pubmed/21887413

Jelonek K, Gdowicz-Kłosok A, Pietrowska P, Borkowska M, Korfanty J, Rzeszowska-Wolny J, Widłak P (2010): Association between single-nucleotide polymorphisms of selected genes involved in the response to DNA damage and risk of colon, head and neck, and breast cancers in a Polish population. J. Appl. Genet 51 : 343-352.

http://www.ncbi.nlm.nih.gov/pubmed/20720310

Horak S, Olejek A, Widłak P (2007): Sperm DNA adducts impair fertilization during ICSI but not during IVF. Folia Histochem. Cytobiol. 45 , suppl. 1: S99-S104.

http://www.ncbi.nlm.nih.gov/pubmed/18292844

Horak S, Polańska J, Widłak P (2003): High levels of bulky DNA adducts in human sperm correlate with impaired fertility. Acta Biochim. Polon 50 : 197-203.

http://www.ncbi.nlm.nih.gov/pubmed/12673360

Horak S, Polańska J, Widłak P (2003): Bulky DNA adducts in human sperm: relationship with fertility, semen quality, smoking, and environmental factors. Mutat. Res. 537 : 53-65.

http://www.ncbi.nlm.nih.gov/pubmed/12742507

Rzeszowska-Wolny J, Polańska J, Pietrowska M, Palyvoda O, Jaworska J, Butkiewicz D, Hancock R (2005):. Influence of polymorphisms in DNA repair genes XPD, XRCC1 and MGMT on DNA damage induced by gamma radiation and its repair in lymphocytes in vitro. Radiat. Res164:132-140.

http://www.ncbi.nlm.nih.gov/pubmed/16038584

Walaszczyk A, Pietrowska M (2009): Kardiotoksyczność promieniowania jonizującego. w: Na Pograniczu Chemii i Biologii, tom. XXII, str. 347-369. Wyd. UAM, Poznań.

Widłak P (2002): Struktura chromatyny a powstawanie i naprawa uszkodzeń DNA. Kosmos 51: 5-12.

Widłak P (2000): The DNA damage-induced cell cycle checkpoints. J. Theor. Med2: 273-243.

Widłak P (2000): Naprawa dwuniciowych pęknięć DNA indukowanych przez promieniowanie jonizujące. Postępy Higieny i Medycyny Doświadczalnej 54: 133-148.

 

Chromatin and gene regulation

Piccinni E, Chełstowska A, Hanus J, Widłak P , Loreti S, Tata AM, Augusti-Tocco G, Bianchi MM, Negri R (2011): Direct interaction of Gas41 and Myc encoded by amplified genes in nervous system tumours. Acta Biochim. Polon58: 529-534.

http://www.ncbi.nlm.nih.gov/pubmed/22068108

Liu Z, Widłak P, Zou Y, Xiao F, Oh M, Li S, Chang MY, Shay JW, Garrard WT (2006): A recombination silencer that specifies heterochromatin positioning and Ikaros association in the immunoglobulin k locus. Immunity 24: 405-415.

http://www.ncbi.nlm.nih.gov/pubmed/16618599

Widłak W, Widłak P (2004): MAR/SAR elements flank the rat hst70 gene transcription unit. Cell. Mol. Biol. Lett 9: 123-133.

http://www.ncbi.nlm.nih.gov/pubmed/15048156

Fedorov A, Lukyanov D, Rogoliński J, Widłak P, Podgornaya O, Rzeszowska-Wolny J (2004): The nuclear protein p30 interacts with a nuclear matrix attachment region from the rat genome. Cell. Mol. Biol. Lett 9: 153-165.

http://www.ncbi.nlm.nih.gov/pubmed/15048159

Widłak P (2004): DNA microarrays, a novel approach in studies of chromatin structure. Acta Biochim. Polon 51: 1-8.

http://www.ncbi.nlm.nih.gov/pubmed/15094821

Weil MR, Widłak P, Minna JD, Garner HR (2004) Global survey of chromatin accessibility using DNA microarrays. Genome Res 14: 1374-1381.

http://www.ncbi.nlm.nih.gov/pubmed/15231753

Widłak P, Fujarewicz K (2003): The analysis of chromatin condensation state and transcriptional activity using DNA microarrays. Journal of Medical Informatics and Technologies. 6: IP13-IP19.

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